TY - JOUR
T1 - Analytical epigenetics
T2 - single-molecule optical detection of DNA and histone modifications
AU - Heck, Christian
AU - Michaeli, Yael
AU - Bald, Ilko
AU - Ebenstein, Yuval
N1 - Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2019/2
Y1 - 2019/2
N2 - The field of epigenetics describes the relationship between genotype and phenotype, by regulating gene expression without changing the canonical base sequence of DNA. It deals with molecular genomic information that is encoded by a rich repertoire of chemical modifications and molecular interactions. This regulation involves DNA, RNA and proteins that are enzymatically tagged with small molecular groups that alter their physical and chemical properties. It is now clear that epigenetic alterations are involved in development and disease, and thus, are the focus of intensive research. The ability to record epigenetic changes and quantify them in rare medical samples is critical for next generation diagnostics. Optical detection offers the ultimate single-molecule sensitivity and the potential for spectral multiplexing. Here we review recent progress in ultrasensitive optical detection of DNA and histone modifications.
AB - The field of epigenetics describes the relationship between genotype and phenotype, by regulating gene expression without changing the canonical base sequence of DNA. It deals with molecular genomic information that is encoded by a rich repertoire of chemical modifications and molecular interactions. This regulation involves DNA, RNA and proteins that are enzymatically tagged with small molecular groups that alter their physical and chemical properties. It is now clear that epigenetic alterations are involved in development and disease, and thus, are the focus of intensive research. The ability to record epigenetic changes and quantify them in rare medical samples is critical for next generation diagnostics. Optical detection offers the ultimate single-molecule sensitivity and the potential for spectral multiplexing. Here we review recent progress in ultrasensitive optical detection of DNA and histone modifications.
UR - http://www.scopus.com/inward/record.url?scp=85054769368&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2018.09.006
DO - 10.1016/j.copbio.2018.09.006
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AN - SCOPUS:85054769368
SN - 0958-1669
VL - 55
SP - 151
EP - 158
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
ER -