Analysis of ultra acidic proteins by the use of anodic acidic gels

Kristina Hempel, Ran Rosen, Dörte Becher, Knut Büttner, Michael Hecker, Eliora Z. Ron

Research output: Contribution to journalArticlepeer-review

Abstract

Ultra acidic proteins, generated by posttranslational modifications, are becoming increasingly important due to recent evidence showing their function as regulatory elements or as intermediates in degradation pathways in bacteria. Such proteins are important in neurodegenerative diseases and embryonic development, and they include the Alzheimer-related tau (τ) protein (resulting from posttranslational modifications) and the phosphor-storage embryonic proteins. The ultra acidic proteins are difficult to study because standard two-dimensional gel electrophoresis is inadequate for their analysis. Here we describe a novel electrophoresis system of anodic acidic gels that can replace isoelectric focusing as the first dimension of separation in two-dimensional electrophoresis. The system is based on a sodium acetate buffer (pH 4.6), is compatible with traditional stains (e.g., Coomassie blue) as well as novel fluorescent dyes (e.g., Pro-Q Diamond), and is quantitative for the analysis of ultra acidic proteins. The anodic acidic gels were used for the functional classification of the ultra acidic part of the Bacillus subtilis proteome, showing significant improvement over traditional two-dimensional electrophoresis.

Original languageEnglish
Pages (from-to)208-214
Number of pages7
JournalAnalytical Biochemistry
Volume385
Issue number2
DOIs
StatePublished - 15 Feb 2009

Keywords

  • Acidic proteins
  • Posttranslational modifications
  • Two-dimensional gel electrophoresis

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