Analysis of the kinetics of lipid peroxidation in terms of characteristic time-points

Ilya Pinchuk*, Dov Lichtenberg

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

Abstract

Measuring peroxidation of aggregated lipids in model systems (liposomes, micelles, emulsions or microemulsions) as well as in samples of biological origin ex vivo (isolated lipoproteins, blood sera or plasma) is widely used in medical and biological investigations, to evaluate the oxidative stress, antioxidants' efficiency and lipid oxidizability in different pathophysiological states. To avoid possible artifacts, such investigations must be based on the time course of peroxidation (i.e. on kinetic studies). To be able to compare complex kinetic profiles, it is important to characterize them in terms of mechanistically meaningful and experimentally unequivocal parameters. In this review, we characterize the typically observed continuous kinetic profiles in terms of a limited number of characteristic time-points (both commonly used and additional time-points and their combinations) that can be derived from experimental time-dependencies. The meaning of each of the experimentally observed characteristic parameters is presented in terms of rate constants and concentrations, derived on the basis of mechanistic considerations. Theoretical expressions for these characteristic parameters are based on a model that includes both the inhibited peroxidation and the uninhibited peroxidation occurring after consumption of the antioxidant(s). Comparison between theoretically predicted dependencies and experimental data support our treatment considered with special emphasis on transition metals-induced peroxidation of lipoproteins.

Original languageEnglish
Pages (from-to)63-76
Number of pages14
JournalChemistry and Physics of Lipids
Volume178
DOIs
StatePublished - Feb 2014

Keywords

  • Hydroperoxides
  • LDL peroxidation kinetics
  • Lag
  • Lipid oxidation

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