TY - JOUR
T1 - Analysis of quinocide in unprocessed primaquine diphosphate and primaquine diphosphate tablets using gas chromatography-mass spectrometry with supersonic molecular beams
AU - Brondz, Ilia
AU - Fialkov, Alexander B.
AU - Amirav, Aviv
N1 - Funding Information:
The authors are grateful to Jon Reierstad, Technical Department, University of Oslo, Norway, for technical assistance. This research was financially supported by Jupiter AS Norway, the Israel Science Foundation (grant no. 1172/07), the James Franck Center for Laser Matter Interaction Research and the Ministry of Science, Culture and Sport of the state of Israel and FZK forschungszentrum Karlsruhe.
PY - 2009/1/30
Y1 - 2009/1/30
N2 - Malaria is one of the most widespread and deadly diseases on the planet. Every year, about 500 million new cases are diagnosed, and the annual death toll is about 3 million. Primaquine has strong antiparasitic effects against gametocytes and can therefore prevent the spread of the parasite from treated patients to mosquitoes. It is also used in radical cures and prevents relapse. Consequently, primaquine is an often-used drug. In this study the separation of unprocessed primaquine from the contaminant quinocide based on gas chromatography-mass spectrometry with supersonic molecular beam (SMB) is presented and 7.5 mg primaquine diphosphate tablets were analyzed. We present a novel method for fast determination of quinocide which is an isomer of primaquine as the main contaminant in unprocessed primaquine and in its medical form as tablets by gas chromatography-mass spectrometry with SMB (also named supersonic GC-MS). Supersonic GC-MS provides enhanced molecular ion without any ion source related peak tailing plus extended range of compounds amenable for GC-MS analysis. In addition, major isomer mass spectral effects were revealed in the mass spectra of primaquine and quinocide which facilitated the unambiguous identification of quinocide in primaquine tablets. Fast GC-MS analysis is demonstrated with less then 2 min elution time of the drug and its main contaminants. Crown
AB - Malaria is one of the most widespread and deadly diseases on the planet. Every year, about 500 million new cases are diagnosed, and the annual death toll is about 3 million. Primaquine has strong antiparasitic effects against gametocytes and can therefore prevent the spread of the parasite from treated patients to mosquitoes. It is also used in radical cures and prevents relapse. Consequently, primaquine is an often-used drug. In this study the separation of unprocessed primaquine from the contaminant quinocide based on gas chromatography-mass spectrometry with supersonic molecular beam (SMB) is presented and 7.5 mg primaquine diphosphate tablets were analyzed. We present a novel method for fast determination of quinocide which is an isomer of primaquine as the main contaminant in unprocessed primaquine and in its medical form as tablets by gas chromatography-mass spectrometry with SMB (also named supersonic GC-MS). Supersonic GC-MS provides enhanced molecular ion without any ion source related peak tailing plus extended range of compounds amenable for GC-MS analysis. In addition, major isomer mass spectral effects were revealed in the mass spectra of primaquine and quinocide which facilitated the unambiguous identification of quinocide in primaquine tablets. Fast GC-MS analysis is demonstrated with less then 2 min elution time of the drug and its main contaminants. Crown
KW - Anti-malaria drug
KW - Contaminations in primaquine tablets
KW - GC-MS
KW - Isomer analysis
KW - Primaquine
KW - Quinocide
KW - Supersonic GC-MS
KW - Supersonic molecular beam
UR - http://www.scopus.com/inward/record.url?scp=58149125117&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2008.11.043
DO - 10.1016/j.chroma.2008.11.043
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AN - SCOPUS:58149125117
SN - 0021-9673
VL - 1216
SP - 824
EP - 829
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 5
ER -
