TY - CHAP
T1 - Analysis of microRNA Regulation in Single Cells
AU - Liu, Wendao
AU - Shomron, Noam
N1 - Publisher Copyright:
© 2021, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2021
Y1 - 2021
N2 - MicroRNAs (miRNAs) regulate gene expression by binding to mRNAs. Consequently, they reduce target gene expression levels and expression variability, also known as “noise.” Single-cell RNA sequencing (scRNA-seq) technology has been used to study miRNA and mRNA expression in single cells, and has demonstrated its strength in quantifying cell-to-cell variation. Here we describe how to investigate miRNA regulation using data with both mRNA and miRNA expression in single cell format. We show that miRNAs reduce the expression levels and also expression noise of target genes in single cells. Finally, we also discuss potential improvements in experimental design and computational analysis of scRNA-seq in order to reduce or partition the technical noise.
AB - MicroRNAs (miRNAs) regulate gene expression by binding to mRNAs. Consequently, they reduce target gene expression levels and expression variability, also known as “noise.” Single-cell RNA sequencing (scRNA-seq) technology has been used to study miRNA and mRNA expression in single cells, and has demonstrated its strength in quantifying cell-to-cell variation. Here we describe how to investigate miRNA regulation using data with both mRNA and miRNA expression in single cell format. We show that miRNAs reduce the expression levels and also expression noise of target genes in single cells. Finally, we also discuss potential improvements in experimental design and computational analysis of scRNA-seq in order to reduce or partition the technical noise.
KW - Gene expression level
KW - Gene expression noise
KW - Single-cell data denoising
KW - Single-cell sequencing
KW - miRNA regulation
UR - http://www.scopus.com/inward/record.url?scp=85102042379&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-1103-6_18
DO - 10.1007/978-1-0716-1103-6_18
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C2 - 33606267
AN - SCOPUS:85102042379
T3 - Methods in Molecular Biology
SP - 339
EP - 354
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -