TY - JOUR
T1 - Analysis of different glycosylation states in IgG subclasses
AU - Keusch, Jeremy
AU - Levy, Yair
AU - Shoenfeld, Yehuda
AU - Youinou, Pierre
N1 - Funding Information:
This study was supportedb y the Associationd e Recherchesu r la PolyarthritReh umatode.
PY - 1996/8/30
Y1 - 1996/8/30
N2 - Altered IgG glycosylation affects certain immunological activities of human IgG. Enzyme-linked lectin assays (ELLA) were developed for detecting the glycosylation on IgG and its individual subclasses in sera from healthy controls. Biotinylated Sambucus nigra, Ricinus communis agglutinin I and Bandeiraea simplificolia II were used to detect the terminal sialic acid (SA), galactose (Gal) and N-acetylglucosamine (GlcNAc) sugar residues, respectively on the captured IgG. A mild oxidation step of the anti-IgG-coated plates obviated background reaction with the lectins. Terminal glycosylation varied significantly with age. The old age group (> 65 years) had less SA in IgG1, and IgG2, when compared to young (0-19 years) and adult (20-39 years) groups, respectively. Also the old age group had less Gal in IgG2, lgG3 and IgG4 subclasses compared to the adult group, and to the young group in the case of IgG3. This ELLA system may be a valuable tool in the detection of glycosylation disorders in patients' sera.
AB - Altered IgG glycosylation affects certain immunological activities of human IgG. Enzyme-linked lectin assays (ELLA) were developed for detecting the glycosylation on IgG and its individual subclasses in sera from healthy controls. Biotinylated Sambucus nigra, Ricinus communis agglutinin I and Bandeiraea simplificolia II were used to detect the terminal sialic acid (SA), galactose (Gal) and N-acetylglucosamine (GlcNAc) sugar residues, respectively on the captured IgG. A mild oxidation step of the anti-IgG-coated plates obviated background reaction with the lectins. Terminal glycosylation varied significantly with age. The old age group (> 65 years) had less SA in IgG1, and IgG2, when compared to young (0-19 years) and adult (20-39 years) groups, respectively. Also the old age group had less Gal in IgG2, lgG3 and IgG4 subclasses compared to the adult group, and to the young group in the case of IgG3. This ELLA system may be a valuable tool in the detection of glycosylation disorders in patients' sera.
KW - Enzyme-linked lectin assay
KW - Glycosylation
KW - IgG subclasses
KW - Lectins
UR - https://www.scopus.com/pages/publications/0030606920
U2 - 10.1016/0009-8981(96)06326-7
DO - 10.1016/0009-8981(96)06326-7
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AN - SCOPUS:0030606920
SN - 0009-8981
VL - 252
SP - 147
EP - 158
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 2
ER -