An oligoribonucleotide polymerase from SV40-infected cells with properties of a primase

Gabriel Kaufmann; Hedda Hoffman Falk

doi:10.1093/nar/10.7.2309

An oligoribonucleotide polymerase from SV40-infected cells with properties of a primase

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Abstract

A transient decaribonucleotide (iRNA) is covalently linked to nascent eukaryotic DNA chains at their 5′ end. Searching for the putative iRNA polymerase (primase), we detected in extracts from SV40-infected cells a DNA-dependent incorporation of UMP residues from UTP into free and DNA linked deca- or similarly sized ribonucleotidea. Denatured salmon sperm DNA served as the standard template in this reaction. SV40 FIII DNA was also an effective template , SV40 FII DNA was ineffective while FT yielded mainly free decaribonucleotides. The incorporation depended on the other rNTPs and was resistant to high concentrations of α-amanitin and rifamycin AF/013, drugs inhibitory to RNA polymerases I, II and III. The results implicate the decaribonucleotide polymerase in the priming of nascent DNA chains and suggest that the unique size of iRNA is encoded within its primase.

Original language	English
Pages (from-to)	2309-2321
Number of pages	13
Journal	Nucleic Acids Research
Volume	10
Issue number	7
DOIs	https://doi.org/10.1093/nar/10.7.2309
State	Published - 10 Apr 1982
Externally published	Yes

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title = "An oligoribonucleotide polymerase from SV40-infected cells with properties of a primase",

abstract = "A transient decaribonucleotide (iRNA) is covalently linked to nascent eukaryotic DNA chains at their 5′ end. Searching for the putative iRNA polymerase (primase), we detected in extracts from SV40-infected cells a DNA-dependent incorporation of UMP residues from UTP into free and DNA linked deca- or similarly sized ribonucleotidea. Denatured salmon sperm DNA served as the standard template in this reaction. SV40 FIII DNA was also an effective template , SV40 FII DNA was ineffective while FT yielded mainly free decaribonucleotides. The incorporation depended on the other rNTPs and was resistant to high concentrations of α-amanitin and rifamycin AF/013, drugs inhibitory to RNA polymerases I, II and III. The results implicate the decaribonucleotide polymerase in the priming of nascent DNA chains and suggest that the unique size of iRNA is encoded within its primase.",

author = "Gabriel Kaufmann and Falk, {Hedda Hoffman}",

note = "Funding Information: ACKNOWLEDGEMENTS We thank V. Daniel, G. Dinter-Gottlieb and S. Eisenberg for discussions, T. Yagura and I. R. Lehman for communicating their results, N. R. Kallenbach for the gift of oligoadenylates and H. Todd for aphidicolin. This work was supported by grants from the US-Israel Binational Science Foundation, Jerusalem. G. K. was an incumbent of the Alan Dixon Career Development Chair.",

year = "1982",

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doi = "10.1093/nar/10.7.2309",

language = "אנגלית",

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AU - Falk, Hedda Hoffman

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PY - 1982/4/10

Y1 - 1982/4/10

N2 - A transient decaribonucleotide (iRNA) is covalently linked to nascent eukaryotic DNA chains at their 5′ end. Searching for the putative iRNA polymerase (primase), we detected in extracts from SV40-infected cells a DNA-dependent incorporation of UMP residues from UTP into free and DNA linked deca- or similarly sized ribonucleotidea. Denatured salmon sperm DNA served as the standard template in this reaction. SV40 FIII DNA was also an effective template , SV40 FII DNA was ineffective while FT yielded mainly free decaribonucleotides. The incorporation depended on the other rNTPs and was resistant to high concentrations of α-amanitin and rifamycin AF/013, drugs inhibitory to RNA polymerases I, II and III. The results implicate the decaribonucleotide polymerase in the priming of nascent DNA chains and suggest that the unique size of iRNA is encoded within its primase.

AB - A transient decaribonucleotide (iRNA) is covalently linked to nascent eukaryotic DNA chains at their 5′ end. Searching for the putative iRNA polymerase (primase), we detected in extracts from SV40-infected cells a DNA-dependent incorporation of UMP residues from UTP into free and DNA linked deca- or similarly sized ribonucleotidea. Denatured salmon sperm DNA served as the standard template in this reaction. SV40 FIII DNA was also an effective template , SV40 FII DNA was ineffective while FT yielded mainly free decaribonucleotides. The incorporation depended on the other rNTPs and was resistant to high concentrations of α-amanitin and rifamycin AF/013, drugs inhibitory to RNA polymerases I, II and III. The results implicate the decaribonucleotide polymerase in the priming of nascent DNA chains and suggest that the unique size of iRNA is encoded within its primase.

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JO - Nucleic Acids Research

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An oligoribonucleotide polymerase from SV40-infected cells with properties of a primase

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