TY - JOUR
T1 - An internal standard for the determination of buoyant density of RNA in CsCl density gradient
AU - Daniel, Ezra
AU - Banin, Dan
PY - 1970/12/14
Y1 - 1970/12/14
N2 - A method is described in which RNA is banded in a CsCl density gradient under conditions where solid CsCl is present at the bottom of the cell. The density of the solution at the interface between solution and solid salt serves as a reference for the calibration of densities anywhere in the liquid column. The buoyant density of MS2 RNA, banded in aqueous CsCl at 40°;, was determined in this way and found to be in agreement with the known value for this molecule. Aqueous CsCl solutions are not sufficiently dense to band RNA at room temperature. It is demonstrated that substitution of 2H2O for H2O as solvent for CsCl makes the banding of RNA at 25° possible.
AB - A method is described in which RNA is banded in a CsCl density gradient under conditions where solid CsCl is present at the bottom of the cell. The density of the solution at the interface between solution and solid salt serves as a reference for the calibration of densities anywhere in the liquid column. The buoyant density of MS2 RNA, banded in aqueous CsCl at 40°;, was determined in this way and found to be in agreement with the known value for this molecule. Aqueous CsCl solutions are not sufficiently dense to band RNA at room temperature. It is demonstrated that substitution of 2H2O for H2O as solvent for CsCl makes the banding of RNA at 25° possible.
UR - http://www.scopus.com/inward/record.url?scp=0014945801&partnerID=8YFLogxK
U2 - 10.1016/0005-2787(70)90564-2
DO - 10.1016/0005-2787(70)90564-2
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AN - SCOPUS:0014945801
SN - 0005-2787
VL - 224
SP - 311
EP - 318
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 2
ER -