An interactive reference framework for modeling a dynamic immune system

Matthew H. Spitzer, Pier Federico Gherardini, Gabriela K. Fragiadakis, Nupur Bhattacharya, Robert T. Yuan, Andrew N. Hotson, Rachel Finck, Yaron Carmi, Eli R. Zunder, Wendy J. Fantl, Sean C. Bendall, Edgar G. Engleman, Garry P. Nolan

Research output: Contribution to journalArticlepeer-review

Abstract

Immune cells function in an interacting hierarchy that coordinates the activities of various cell types according to genetic and environmental contexts. We developed graphical approaches to construct an extensible immune reference map from mass cytometry data of cells from different organs, incorporating landmark cell populations as flags on the map to compare cells from distinct samples. The maps recapitulated canonical cellular phenotypes and revealed reproducible, tissue-specific deviations. The approach revealed influences of genetic variation and circadian rhythms on immune system structure, enabled direct comparisons of murine and human blood cell phenotypes, and even enabled archival fluorescence-based flow cytometry data to be mapped onto the reference framework. This foundational reference map provides a working definition of systemic immune organization to which new data can be integrated to reveal deviations driven by genetics, environment, or pathology.

Original languageEnglish
Article number1259425
JournalScience
Volume349
Issue number6244
DOIs
StatePublished - 10 Jul 2015
Externally publishedYes

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