An initial blueprint for myogenic differentiation

Alexandre Blais, Mary Tsikitis, Diego Acosta-Alvear, Roded Sharan, Yuval Kluger, Brian David Dynlacht

Research output: Contribution to journalArticlepeer-review


We have combined genome-wide transcription factor binding and expression profiling to assemble a regulatory network controlling the myogenic differentiation program in mammalian cells. We identified a cadre of overlapping and distinct targets of the key myogenic regulatory factors (MRFs)-MyoD and myogenin-and Myocyte Enhancer Factor 2 (MEF2). We discovered that MRFs and MEF2 regulate a remarkably extensive array of transcription factor genes that propagate and amplify the signals initiated by MRFs. We found that MRFs play an unexpectedly wide-ranging role in directing the assembly and usage of the neuromuscular junction. Interestingly, these factors also prepare myoblasts to respond to diverse types of stress. Computational analyses identified novel combinations of factors that, depending on the differentiation state, might collaborate with MRFs. Our studies suggest unanticipated biological insights into muscle development and highlight new directions for further studies of genes involved in muscle repair and responses to stress and damage.

Original languageEnglish
Pages (from-to)553-569
Number of pages17
JournalGenes and Development
Issue number5
StatePublished - 1 Mar 2005


  • ChIP-on-chip
  • Myogenesis
  • Transcriptional regulation network


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