Abstract
Sixty-six bone-marrow specimens, derived from patients with hematological and non-hematological disorders, were processed for cytogenetic analysis. Modifications of various parameters of the standard (direct) culture procedure were investigated and the quality of the preparations determined. As a result of these experiments, an improved culture technique was developed that yielded significantly better quality chromosome preparations. This method is based on a short incubation (25-min) of the bone-marrow specimen, immediately following aspiration, in a solution containing both hypotonic KCl and colcemid and completely omits the use of tissue culture medium.
| Original language | English |
|---|---|
| Pages (from-to) | 510-515 |
| Number of pages | 6 |
| Journal | In Vitro |
| Volume | 14 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1978 |
| Externally published | Yes |
Keywords
- bone marrow
- chromosomes
- culture technique
- hematological disorders
- karyotypes