An improved method for isolation and purification of soluble tumor-associated proteins from the serum of cancer patients

B. Sandler; P. Smirnoff; H. Ben-Hur; R. Zusman; I. Zusman

An improved method for isolation and purification of soluble tumor-associated proteins from the serum of cancer patients

B. Sandler, P. Smirnoff, H. Ben-Hur, R. Zusman, I. Zusman^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A simple, rapid and effective method for the isolation of the soluble tumor-associated antigens from cancer patient serum is described. The method is based on the use of gel fiberglass (GFG) affinity chromatography and BioGel P-60 columns which enable isolation of single proteins from blood sera. GFG is a new affinity biosensor for isolation of human s53 antigen using rabbit anti-s53 IgG. The described method may be used for the purification of different organ-specific antigens which are of interest in biology and medicine.

Original language	English
Pages (from-to)	55-58
Number of pages	4
Journal	Experimental Oncology
Volume	21
Issue number	1
State	Published - Mar 1999
Externally published	Yes

Keywords

Affinity chromatography
Colon cancer
Gel fiberglass
IgG
Tumor-associated antigens

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This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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abstract = "A simple, rapid and effective method for the isolation of the soluble tumor-associated antigens from cancer patient serum is described. The method is based on the use of gel fiberglass (GFG) affinity chromatography and BioGel P-60 columns which enable isolation of single proteins from blood sera. GFG is a new affinity biosensor for isolation of human s53 antigen using rabbit anti-s53 IgG. The described method may be used for the purification of different organ-specific antigens which are of interest in biology and medicine.",

keywords = "Affinity chromatography, Colon cancer, Gel fiberglass, IgG, Tumor-associated antigens",

author = "B. Sandler and P. Smirnoff and H. Ben-Hur and R. Zusman and I. Zusman",

year = "1999",

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T1 - An improved method for isolation and purification of soluble tumor-associated proteins from the serum of cancer patients

AU - Sandler, B.

AU - Smirnoff, P.

AU - Ben-Hur, H.

AU - Zusman, R.

AU - Zusman, I.

PY - 1999/3

Y1 - 1999/3

N2 - A simple, rapid and effective method for the isolation of the soluble tumor-associated antigens from cancer patient serum is described. The method is based on the use of gel fiberglass (GFG) affinity chromatography and BioGel P-60 columns which enable isolation of single proteins from blood sera. GFG is a new affinity biosensor for isolation of human s53 antigen using rabbit anti-s53 IgG. The described method may be used for the purification of different organ-specific antigens which are of interest in biology and medicine.

AB - A simple, rapid and effective method for the isolation of the soluble tumor-associated antigens from cancer patient serum is described. The method is based on the use of gel fiberglass (GFG) affinity chromatography and BioGel P-60 columns which enable isolation of single proteins from blood sera. GFG is a new affinity biosensor for isolation of human s53 antigen using rabbit anti-s53 IgG. The described method may be used for the purification of different organ-specific antigens which are of interest in biology and medicine.

KW - Affinity chromatography

KW - Colon cancer

KW - Gel fiberglass

KW - IgG

KW - Tumor-associated antigens

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AN - SCOPUS:0345734699

SN - 0204-3564

VL - 21

SP - 55

EP - 58

JO - Experimental Oncology

JF - Experimental Oncology

IS - 1

ER -

An improved method for isolation and purification of soluble tumor-associated proteins from the serum of cancer patients

Abstract

Keywords

UN SDGs

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