An improved method for isolation and purification of soluble tumor-associated proteins from the serum of cancer patients

B. Sandler, P. Smirnoff, H. Ben-Hur, R. Zusman, I. Zusman

Research output: Contribution to journalArticlepeer-review

Abstract

A simple, rapid and effective method for the isolation of the soluble tumor-associated antigens from cancer patient serum is described. The method is based on the use of gel fiberglass (GFG) affinity chromatography and BioGel P-60 columns which enable isolation of single proteins from blood sera. GFG is a new affinity biosensor for isolation of human s53 antigen using rabbit anti-s53 IgG. The described method may be used for the purification of different organ-specific antigens which are of interest in biology and medicine.

Original languageEnglish
Pages (from-to)55-58
Number of pages4
JournalExperimental Oncology
Volume21
Issue number1
StatePublished - Mar 1999
Externally publishedYes

Keywords

  • Affinity chromatography
  • Colon cancer
  • Gel fiberglass
  • IgG
  • Tumor-associated antigens

Fingerprint

Dive into the research topics of 'An improved method for isolation and purification of soluble tumor-associated proteins from the serum of cancer patients'. Together they form a unique fingerprint.

Cite this