TY - JOUR
T1 - An evolutionarily conserved mechanism for controlling the efficiency of protein translation
AU - Tuller, Tamir
AU - Carmi, Asaf
AU - Vestsigian, Kalin
AU - Navon, Sivan
AU - Dorfan, Yuval
AU - Zaborske, John
AU - Pan, Tao
AU - Dahan, Orna
AU - Furman, Itay
AU - Pilpel, Yitzhak
N1 - Funding Information:
We thank the Pilpel lab and Chaim Kahana, Ran Kafri, Ron Milo, Uri Alon, Michael Springer, Jeremy Gunawardena, Eytan Ruppin, and Martin Kupiec for stimulating discussions and Orna Man for critically reading the manuscript. We thank the “Ideas” program of the European Research Council, EMBRACE Network of Excellence grant of the European Commission within its FP6 Programme, and the Ben May Charitable Trust for grant support. T.T. is a Koshland Scholar at the Weizmann Institute of Science.
PY - 2010/4
Y1 - 2010/4
N2 - Recent years have seen intensive progress in measuring protein translation. However, the contributions of coding sequences to the efficiency of the process remain unclear. Here, we identify a universally conserved profile of translation efficiency along mRNAs computed based on adaptation between coding sequences and the tRNA pool. In this profile, the first ∼30-50 codons are, on average, translated with a low efficiency. Additionally, in eukaryotes, the last ∼50 codons show the highest efficiency over the full coding sequence. The profile accurately predicts position-dependent ribosomal density along yeast genes. These data suggest that translation speed and, as a consequence, ribosomal density are encoded by coding sequences and the tRNA pool. We suggest that the slow " ramp" at the beginning of mRNAs serves as a late stage of translation initiation, forming an optimal and robust means to reduce ribosomal traffic jams, thus minimizing the cost of protein expression.
AB - Recent years have seen intensive progress in measuring protein translation. However, the contributions of coding sequences to the efficiency of the process remain unclear. Here, we identify a universally conserved profile of translation efficiency along mRNAs computed based on adaptation between coding sequences and the tRNA pool. In this profile, the first ∼30-50 codons are, on average, translated with a low efficiency. Additionally, in eukaryotes, the last ∼50 codons show the highest efficiency over the full coding sequence. The profile accurately predicts position-dependent ribosomal density along yeast genes. These data suggest that translation speed and, as a consequence, ribosomal density are encoded by coding sequences and the tRNA pool. We suggest that the slow " ramp" at the beginning of mRNAs serves as a late stage of translation initiation, forming an optimal and robust means to reduce ribosomal traffic jams, thus minimizing the cost of protein expression.
KW - Proteins
UR - http://www.scopus.com/inward/record.url?scp=77951715627&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2010.03.031
DO - 10.1016/j.cell.2010.03.031
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AN - SCOPUS:77951715627
SN - 0092-8674
VL - 141
SP - 344
EP - 354
JO - Cell
JF - Cell
IS - 2
ER -