The upstream region of the metA gene in Escherichia coli contains two promoters. We have identified by lacZ fusion an additional promoter in this region, and showed that it is transcribed in the opposite orientation from the metA gene. The putative translation product corresponds to a peptide of 147 amino acids - ORF19 by molecular mass. This peptide is probably not essential for growth, as an insertion mutant is viable.
- Escherichia coli
- Transcriptional regulation