TY - JOUR
T1 - Amplified telomerase analysis by using rotating magnetic particles
T2 - The rapid and sensitive detection of cancer cells
AU - Weizmann, Yossi
AU - Patolsky, Fernando
AU - Katz, Eugenii
AU - Willner, Itamar
PY - 2004/7/5
Y1 - 2004/7/5
N2 - A highly sensitive telomerase detection method that involves amplified telomerase analysis and the use of rotating magnetic particles has been developed. Magnetic particles, functionalized with a primer (1) that is recognized by telomerase, are mixed with a nucleotide mixture that includes biotinylated-dUTP, and telomerase-induced elongation of the primers proceeds with simultaneous biotin incorporation. Avidin-Horseradish peroxidase conjugate, coupled to biotin labels, yields the biocatalytic functional particles. Mixing the resulting particles with naphthoquinone-modified magnetic particles enables the optoelectronic detection of telomerase. Attraction of the magnetic particles to an electrode, followed by rotation of the particles, causes the electrocatalytic reduction of O2 to H2O2 and HRP-catalyzed oxidation of luminol (3); this results in chemilumunescence. The intensity of the emitted light depends on the telomerase content of the sample and the rotation speed of the particles. A minimum number of 10 cancer cells could be detected.
AB - A highly sensitive telomerase detection method that involves amplified telomerase analysis and the use of rotating magnetic particles has been developed. Magnetic particles, functionalized with a primer (1) that is recognized by telomerase, are mixed with a nucleotide mixture that includes biotinylated-dUTP, and telomerase-induced elongation of the primers proceeds with simultaneous biotin incorporation. Avidin-Horseradish peroxidase conjugate, coupled to biotin labels, yields the biocatalytic functional particles. Mixing the resulting particles with naphthoquinone-modified magnetic particles enables the optoelectronic detection of telomerase. Attraction of the magnetic particles to an electrode, followed by rotation of the particles, causes the electrocatalytic reduction of O2 to H2O2 and HRP-catalyzed oxidation of luminol (3); this results in chemilumunescence. The intensity of the emitted light depends on the telomerase content of the sample and the rotation speed of the particles. A minimum number of 10 cancer cells could be detected.
KW - Biosensors
KW - Cancer
KW - DNA
KW - Enzymes
KW - Telomerase
UR - http://www.scopus.com/inward/record.url?scp=4544273246&partnerID=8YFLogxK
U2 - 10.1002/cbic.200300820
DO - 10.1002/cbic.200300820
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AN - SCOPUS:4544273246
SN - 1439-4227
VL - 5
SP - 943
EP - 948
JO - ChemBioChem
JF - ChemBioChem
IS - 7
ER -
