Abstract
A novel amplification route for DNA detection based on the deposition of gold on a 10 nm Au-colloid/avidin conjugate label acting as a 'seeding' catalyst, is described. Microgravimetric quartz-crystal-microbalance measurements are employed to transduce the catalyzed deposition of gold on the piezoelectric crystals, Three different DNA detection schemes are described: (i) analysis of a 27-base nucleic acid fragment; (ii) analysis of the entire M13φ DNA (7229 bases); and (iii) detection of a single-base mismatch in a DNA. Ultrasensitive detection of DNA is accomplished by the catalyzed deposition of gold, detection limit ∼ 1 × 10-15 M.
| Original language | English |
|---|---|
| Pages (from-to) | 1502-1504 |
| Number of pages | 3 |
| Journal | The Analyst |
| Volume | 126 |
| Issue number | 9 |
| DOIs | |
| State | Published - 2001 |
| Externally published | Yes |