Amplification of immunological functions by subcutaneous injection of intermediate-high dose interleukin-2 for 2 years after autologous stem cell transplantation in children with stage IV neuroblastoma

Amos Toren, Arnon Nagler, Galit Rozenfeld-Granot, Michal Levanon, Jacqueline Davidson, Bella Bielorai, Chaim Kaplinsky, Dafna Meitar, Mathilda Mandel, Aliza Ackerstein, Ami Ballin, Dina Attias, Miriam Biniaminov, Esther Rosenthal, Frida Brok-Simoni, Gideon Rechavi, Yael Kaufmann

Research output: Contribution to journalArticlepeer-review

Abstract

Background. Immunotherapy given post-autologous stem cell transplantation may eliminate residual tumor cells escaping the conditioning protocol. Methods. Five children suffering from stage IV neuroblastoma were treated by recombinant interleukin-2 (IL-2) post-autologous peripheral blood stem cell transplantation. The patients' peripheral mononuclear cells were monitored for CD3+ and CD56+ levels, their proliferative response and killing of various cell lines targets. Results. An increase in the level of total lymphocytes, mainly due to expansion of T cells, and enhanced proliferative response to phytohemaglutinin were observed. Elevated cytotoxicity against K562 and neuroblastoma target cells was detected in four patients and against K562 targets in one patient. Toxicity included mild thrombocytopenia, and fever in four patients and mild to moderate encephalopathy which necessitated withdrawing one patient from the protocol. Three of five patients studied are alive today, one of them whose IL-2 was stopped, is in relapse. Two patients have died. Conclusions. Immunotherapy with s.c. intermediate-high dose IL-2 is feasible and results in expansion of T cells and in stimulation of killing activity against several targets including in some cases, neuroblastoma tumor cells.

Original languageEnglish
Pages (from-to)1100-1104
Number of pages5
JournalTransplantation
Volume70
Issue number7
DOIs
StatePublished - 15 Oct 2000

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