Amiloride-sensitive epithelial sodium channel subunits are expressed in human and mussel immunocytes

Enzo Ottaviani*, Antonella Franchini, Mauro Mandrioli, Anjana Saxena, Aaron Hanukoglu, Israel Hanukoglu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


In this study, we examined the expression of epithelial Na+ channel (ENaC) subunits in human peripheral blood lymphocytes, human lymph nodes and molluscan immunocytes using non-radioactive in situ hybridization. The results showed that T lymphocytes express the ENaC gamma subunit mRNA, and B lymphocytes the ENaC beta subunit mRNA. Yet, the alpha subunit mRNA was not detected in either cell type. In molluscan immunocytes, all three homologous ENaC subunit mRNAs are present, and these data were also confirmed by RT-PCR and sequencing of the PCR products. These findings show evolutionary conservation of the expression of ENaC subunits in immunocytes of invertebrates to vertebrates. The observed differential expression patterns of ENaC subunits suggest that ENaC function may be regulated differentially in different types of human lymphocytes.

Original languageEnglish
Pages (from-to)395-402
Number of pages8
JournalDevelopmental and Comparative Immunology
Issue number5
StatePublished - 2002


  • ENaC
  • Human T lymphocytes
  • Human lymph nodes
  • In situ hybridization
  • Molluscan immunocytes
  • RT-PCR


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