TY - JOUR
T1 - Amiloride-sensitive epithelial sodium channel subunits are expressed in human and mussel immunocytes
AU - Ottaviani, Enzo
AU - Franchini, Antonella
AU - Mandrioli, Mauro
AU - Saxena, Anjana
AU - Hanukoglu, Aaron
AU - Hanukoglu, Israel
N1 - Funding Information:
Our research was supported by a MURST (Italy) grant and by a grant from the Chief Scientist's Office of the Ministry of Health, Israel.
PY - 2002
Y1 - 2002
N2 - In this study, we examined the expression of epithelial Na+ channel (ENaC) subunits in human peripheral blood lymphocytes, human lymph nodes and molluscan immunocytes using non-radioactive in situ hybridization. The results showed that T lymphocytes express the ENaC gamma subunit mRNA, and B lymphocytes the ENaC beta subunit mRNA. Yet, the alpha subunit mRNA was not detected in either cell type. In molluscan immunocytes, all three homologous ENaC subunit mRNAs are present, and these data were also confirmed by RT-PCR and sequencing of the PCR products. These findings show evolutionary conservation of the expression of ENaC subunits in immunocytes of invertebrates to vertebrates. The observed differential expression patterns of ENaC subunits suggest that ENaC function may be regulated differentially in different types of human lymphocytes.
AB - In this study, we examined the expression of epithelial Na+ channel (ENaC) subunits in human peripheral blood lymphocytes, human lymph nodes and molluscan immunocytes using non-radioactive in situ hybridization. The results showed that T lymphocytes express the ENaC gamma subunit mRNA, and B lymphocytes the ENaC beta subunit mRNA. Yet, the alpha subunit mRNA was not detected in either cell type. In molluscan immunocytes, all three homologous ENaC subunit mRNAs are present, and these data were also confirmed by RT-PCR and sequencing of the PCR products. These findings show evolutionary conservation of the expression of ENaC subunits in immunocytes of invertebrates to vertebrates. The observed differential expression patterns of ENaC subunits suggest that ENaC function may be regulated differentially in different types of human lymphocytes.
KW - ENaC
KW - Human T lymphocytes
KW - Human lymph nodes
KW - In situ hybridization
KW - Molluscan immunocytes
KW - RT-PCR
UR - http://www.scopus.com/inward/record.url?scp=0036198819&partnerID=8YFLogxK
U2 - 10.1016/S0145-305X(01)00097-0
DO - 10.1016/S0145-305X(01)00097-0
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AN - SCOPUS:0036198819
SN - 0145-305X
VL - 26
SP - 395
EP - 402
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
IS - 5
ER -