TY - JOUR
T1 - Alternative promoters in the pst operon of Escherichia coli
AU - Spira, Beny
AU - Aguena, Meire
AU - De Castro Oliveira, Juliana Velasco
AU - Yagil, Ezra
N1 - Funding Information:
Acknowledgments This work was supported by Fundac¸ão de Amparo à Pesquisa do Estado de São Paulo (FAPESP). Meire Aguena was a recipient of FAPESP Ph.D. scholarship 02/04070-9. Juliana Velasco was a recipient of a CNPq undergraduate scholarship. We also thank FAPESP and CNPq for supported travel allowances for Ezra Yagil travel allowances to Brazil. Luiz Gustavo de Almeida performed the determination of Pi concentration in LB and medium A.
PY - 2010/12
Y1 - 2010/12
N2 - The pst operon of Escherichia coli is composed of five genes pstS, pstC, pstA, pstB and phoU, that encode a high-affinity phosphate transport system and a negative regulator of the PHO regulon. Transcription of pst is induced under phosphate shortage and is initiated at the promoter located upstream of the first gene of the operon, pstS. Here, we show by four different technical approaches the existence of additional internal promoters upstream of pstC, pstB and phoU. These promoters are not induced by Pi-limitation and do not possess PHO-box sequences. Plasmids carrying the pst internal genes partially complement chromosomal mutations in their corresponding genes, indicating that they are translated into functional proteins.
AB - The pst operon of Escherichia coli is composed of five genes pstS, pstC, pstA, pstB and phoU, that encode a high-affinity phosphate transport system and a negative regulator of the PHO regulon. Transcription of pst is induced under phosphate shortage and is initiated at the promoter located upstream of the first gene of the operon, pstS. Here, we show by four different technical approaches the existence of additional internal promoters upstream of pstC, pstB and phoU. These promoters are not induced by Pi-limitation and do not possess PHO-box sequences. Plasmids carrying the pst internal genes partially complement chromosomal mutations in their corresponding genes, indicating that they are translated into functional proteins.
KW - Internal promoters
KW - PHO regulon
KW - Pst operon
KW - Transcription start point
UR - http://www.scopus.com/inward/record.url?scp=78650512554&partnerID=8YFLogxK
U2 - 10.1007/s00438-010-0584-x
DO - 10.1007/s00438-010-0584-x
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C2 - 20963440
AN - SCOPUS:78650512554
SN - 1617-4615
VL - 284
SP - 489
EP - 498
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
IS - 6
ER -