Abstract
Mitochondria constitute well-established target for photodynamic action provided that duration of incubation time with hydrophobic photosensitizer is long enough (18-24 hrs) to enable incorporation of the dye into these organelles. It was found that 1 hour incubation of rat cardiocytes with haematoporphyrin dihydrochloride (Hp) followed by low-energy HeNe laser irradiation (ED: 0.3, 1.0 or 3.0 J/cm2), affected mitochondrial membrane potential. The latter was monitored by means of the fast-response fluorescent probe DASPMI and the dynamic video imaging system equipped with frame-grabbing and analyzing software. The time-course of mitochondrial membrane potential decrease exhibited dependence on both: Hp concentration and ED used. In cells incubated with 1 μg/ml Hp a decrease of DASPMI fluorescence occurred during 8-15 mins and was preceded by oscillations of fluorescence intensity. For cells incubated with 100 μg/ml Hp disappearance of discrete pattern of mitochondrial fluorescence was observed 1-2 mins after irradiation.
| Original language | English |
|---|---|
| Pages (from-to) | 146-154 |
| Number of pages | 9 |
| Journal | Proceedings of SPIE - The International Society for Optical Engineering |
| Volume | 3191 |
| DOIs | |
| State | Published - 1997 |
| Externally published | Yes |
| Event | Photochemotherapy: Photodynamic Therapy and Other Modalities III - San Remo, Italy Duration: 4 Sep 1997 → 4 Sep 1997 |
Keywords
- Cardiocytes
- Fluorescent probe
- Haematoporphyrin
- Mitochondrial membrane potential
- Photosensitization