Alpha interferon has no effect on lidocaine metabolism in the rat

E. Melzer*, E. Bardan, I. Ronen, Z. Krepel, S. Bar Meir

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Interferons (IFN) inhibit activity of many isoenzymes of the hepatic microsomal cytochrome P-450 system. This inhibition is species specific. Lidocaine is metabolized by cytochrome P-450 III A 4. We investigated in the rat the effect of rat alpha-IFN on lidocaine elimination and on its extraction by the isolated perfused rat liver. To determine elimination, the femoral artery and vein were cannulated. 24 h later, the conscious rat was given lidocaine through the venous catheter and blood was drawn from the arterial catheter for lidocaine determination every 3 min for 20 min. 7 rats were pre-treated with intramuscular rat alpha-IFN 7.5 × 105 U, 24 h prior to the experiment and another 4 rats were given saline i.m. The lidocaine elimination rate constant was unchanged, 0,065 min-1 and 0.063 min-1 for the control and IFN groups, respectively. To investigate lidocaine extraction, the isolated perfused rat liver was used. Perfusate samples from the portal and hepatic veins were drawn at 2 min intervals for 20 min, and lidocaine extraction determined. Extraction was determined in two groups of 6 rats each. The first group served as control and these rats were injected with saline only, while in the second group, the rats were pre-treated with rat alpha-IFN 7.5 × 105 U. Lidocaine extraction by the isolated perfused rat liver remained unchanged, 97.0 ± 0.7% and 94.0 ± 2.4% in the control and IFN treated groups, respectively. It is concluded that the rat alpha-IFN affects neither the elimination nor the extraction of lidocaine.

Original languageEnglish
Pages (from-to)151-155
Number of pages5
JournalEuropean Journal of Drug Metabolism and Pharmacokinetics
Volume19
Issue number2
DOIs
StatePublished - Jun 1994

Keywords

  • Cytochromes
  • interferon
  • isolated perfused rat liver

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