Allicin inhibits spontaneous and TNF-α induced secretion of proinflammatory cytokines and chemokines from intestinal epithelial cells

Alon Lang, Maor Lahav, Emad Sakhnini, Iris Barshack, Herma H. Fidder, Benjamin Avidan, Eitan Bardan, Rami Hershkoviz, Simon Bar-Meir, Yehuda Chowers

Research output: Contribution to journalArticlepeer-review

Abstract

Background & aims: Allicin, the active substance of fresh crushed garlic has different biological activities and was implicated as an anti-inflammatory agent. Epithelial cells have an important role in intestinal inflammation. The aim of this study was to assess the immunomodulatory effect of allicin on intestinal epithelial cells. Methods: The spontaneous and TNF-α-stimulated secretion of IL-1β, IL-8, IP-10 and MIG from HT-29 and Caco-2 cells was tested with, or without pretreatment with allicin. Cytokine secretion was assessed using ELISA and expression of mRNA was determined by an RNA protection assay. Results: Allicin markedly inhibited the spontaneous and TNF-α-induced secretion of IL-1β, IL-8, IP-10 and MIG from the two different cell lines in a dose-dependent manner and suppressed the expression of IL-8 and IL-1β mRNA levels. In addition, allicin suppressed the degradation of I κ B. No effect on cell viability was noted. Conclusions: These observations indicate that allicin exerts an inhibitory immunomodulatory effect on intestinal epithelial cells and suggest that allicin may have the potential to attenuate intestinal inflammation.

Original languageEnglish
Pages (from-to)1199-1208
Number of pages10
JournalClinical Nutrition
Volume23
Issue number5
DOIs
StatePublished - Oct 2004

Keywords

  • Allicin
  • Chemokines
  • Cytokines
  • Epithelial cells
  • Intestine
  • TNF-α

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