TY - JOUR
T1 - Alkaline phosphatase immuno‐enzymatic technique in the diagnosis of pemphigus vulgaris
AU - TUR, E.
AU - VARDINON, N.
AU - EYLAN, E.
AU - FRISCH, B.
AU - BRENNER, S.
AU - WEINBERG, M.
AU - KRAKOWSKI, A.
PY - 1983/1
Y1 - 1983/1
N2 - Alkaline phosphatase was used in an immuno‐enzymatic procedure to detect tissue‐bound and circulating antibodies in pemphigus vulgaris. Pemphigus antibodies were revealed by a direct method using alkaline phosphatase conjugated goat anti‐human IgG. Cryostat sections were incubated with the specific antiserum, and alkaline phosphatase activity was then revealed histochemically either by Gomori's technique or by the azo dye method. The sections were examined by light microscopy and intercellular staining was demonstrated in the epidermis. The indirect method, in which the patient's serum was incubated with sections of normal skin, gave similar results. Using parallel sections, an immunofluorescent technique was used to demonstrate tissue‐bound and circulating antibodies. The alkaline phosphatase method appeared to be slightly less sensitive than the immunofluorescent method.
AB - Alkaline phosphatase was used in an immuno‐enzymatic procedure to detect tissue‐bound and circulating antibodies in pemphigus vulgaris. Pemphigus antibodies were revealed by a direct method using alkaline phosphatase conjugated goat anti‐human IgG. Cryostat sections were incubated with the specific antiserum, and alkaline phosphatase activity was then revealed histochemically either by Gomori's technique or by the azo dye method. The sections were examined by light microscopy and intercellular staining was demonstrated in the epidermis. The indirect method, in which the patient's serum was incubated with sections of normal skin, gave similar results. Using parallel sections, an immunofluorescent technique was used to demonstrate tissue‐bound and circulating antibodies. The alkaline phosphatase method appeared to be slightly less sensitive than the immunofluorescent method.
UR - http://www.scopus.com/inward/record.url?scp=0020661864&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2133.1983.tb04581.x
DO - 10.1111/j.1365-2133.1983.tb04581.x
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C2 - 6336946
AN - SCOPUS:0020661864
VL - 108
SP - 77
EP - 82
JO - British Journal of Dermatology
JF - British Journal of Dermatology
SN - 0007-0963
IS - 1
ER -