Alkaline phosphatase detection using electrochemical impedance of anti-alkaline phosphatase antibody (Ab354) functionalized silicon-nanowire-forest in phosphate buffer solution

Nofar Mintz Hemed, Annalisa Convertino, Yosi Shacham-Diamand

Research output: Contribution to journalArticlepeer-review

Abstract

This article presents an electrochemical impedance study on the detection of Alkaline Phosphatase (ALP) in phosphate buffer saline (PBS) solution by using anti-ALP functionalized Si nanowires (SiNWs). Electrochemical impedance was measured using a three-electrode cell where the working electrode was a very dense and highly disordered array of Si NWs, e.g. SiNW-forest. This array was grown using plasma enhanced chemical vapor deposition (PECVD) at 350 °C. The NW surface was modified by immobilizing the anti-ALP. A very efficient detection of very low ALP concentrations in PBS solution, ranging from 0.03 U/L to 0.3 U/L (1 nM–10 nM respectively), as well as a high selectivity due to the antibody/antigen interaction were demonstrated. The remarkable sensitivity and selectivity achieved through the combination of SiNW-forest combined electrochemical impedance spectroscopy suggest an effective strategy for real time and quantitative sensing of ALP.

Original languageEnglish
Pages (from-to)809-815
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume259
DOIs
StatePublished - 15 Apr 2018

Keywords

  • Alkaline phosphatase
  • Biosensor
  • Electrochemical impedance spectroscopy
  • Si nanowire
  • Si nanowire-forest

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