TY - JOUR
T1 - Aligned deposition and electrical measurements on single DNA molecules
AU - Eidelshtein, Gennady
AU - Kotlyar, Alexander
AU - Hashemi, Mohtadin
AU - Gurevich, Leonid
N1 - Publisher Copyright:
© 2015 IOP Publishing Ltd.
PY - 2015/11/5
Y1 - 2015/11/5
N2 - A reliable method of deposition of aligned individual dsDNA molecules on mica, silicon, and micro/nanofabricated circuits is presented. Complexes of biotinylated double stranded poly(dG)-poly(dC) DNA with avidin were prepared and deposited on mica and silicon surfaces in the absence of Mg2+ ions. Due to its positive charge, the avidin attached to one end of the DNA anchors the complex to negatively charged substrates. Subsequent drying with a directional gas flow yields DNA molecules perfectly aligned on the surface. In the avidin-DNA complex only the avidin moiety is strongly and irreversibly bound to the surface, while the DNA counterpart interacts with the substrates much more weakly and can be lifted from the surface and realigned in any direction. Using this technique, avidin-DNA complexes were deposited across platinum electrodes on a silicon substrate. Electrical measurements on the deposited DNA molecules revealed linear IV-characteristics and exponential dependence on relative humidity.
AB - A reliable method of deposition of aligned individual dsDNA molecules on mica, silicon, and micro/nanofabricated circuits is presented. Complexes of biotinylated double stranded poly(dG)-poly(dC) DNA with avidin were prepared and deposited on mica and silicon surfaces in the absence of Mg2+ ions. Due to its positive charge, the avidin attached to one end of the DNA anchors the complex to negatively charged substrates. Subsequent drying with a directional gas flow yields DNA molecules perfectly aligned on the surface. In the avidin-DNA complex only the avidin moiety is strongly and irreversibly bound to the surface, while the DNA counterpart interacts with the substrates much more weakly and can be lifted from the surface and realigned in any direction. Using this technique, avidin-DNA complexes were deposited across platinum electrodes on a silicon substrate. Electrical measurements on the deposited DNA molecules revealed linear IV-characteristics and exponential dependence on relative humidity.
KW - AFM
KW - avidin-DNA complex
KW - directional deposition
KW - electrical measurements
UR - http://www.scopus.com/inward/record.url?scp=84947057608&partnerID=8YFLogxK
U2 - 10.1088/0957-4484/26/47/475102
DO - 10.1088/0957-4484/26/47/475102
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AN - SCOPUS:84947057608
SN - 0957-4484
VL - 26
JO - Nanotechnology
JF - Nanotechnology
IS - 47
M1 - 475102
ER -