Aflatoxin B1 mutagenesis, DNA binding, and adduct formation in Salmonella typhimurium

A. A. Stark, J. M. Essigmann, A. L. Demain

Research output: Contribution to journalArticlepeer-review


Salmonella typhimurium strain TM677 was mutagenized with aflatoxin B1 (AFB1) in liquid suspension culture in the presence of a rat liver postmitochondrial supernatant. Forward mutation to 8-azaguanine resistance was measured in the treated cultures and was found to increase linearly with AFB1 concentration. DNA purified from mutagenized cells was analyzed for AFB1 adduct formation by high-pressure liquid chromatography after adduct liberation. AFB1 exposures at 0.16 and 0.32 μM for 35 min produced 15 and 22 AFB1-DNA adducts per genome, respectively, and induced 8-azaguanine-resistant fractions of 4.9 X 10-4 and 9.6 X 10-4. Approximately 70% of the AFB1 bound to DNA was chromatographically identical to 2,3-dihydro-2-(N7-guanyl)-3-hydroxyaflatoxin B1 at the two AFB1 levels used.

Original languageEnglish
Pages (from-to)1343-1347
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number3
StatePublished - 1979
Externally publishedYes


Dive into the research topics of 'Aflatoxin B1 mutagenesis, DNA binding, and adduct formation in Salmonella typhimurium'. Together they form a unique fingerprint.

Cite this