TY - JOUR
T1 - Aflatoxin B1 mutagenesis, DNA binding, and adduct formation in Salmonella typhimurium
AU - Stark, A. A.
AU - Essigmann, J. M.
AU - Demain, A. L.
PY - 1979
Y1 - 1979
N2 - Salmonella typhimurium strain TM677 was mutagenized with aflatoxin B1 (AFB1) in liquid suspension culture in the presence of a rat liver postmitochondrial supernatant. Forward mutation to 8-azaguanine resistance was measured in the treated cultures and was found to increase linearly with AFB1 concentration. DNA purified from mutagenized cells was analyzed for AFB1 adduct formation by high-pressure liquid chromatography after adduct liberation. AFB1 exposures at 0.16 and 0.32 μM for 35 min produced 15 and 22 AFB1-DNA adducts per genome, respectively, and induced 8-azaguanine-resistant fractions of 4.9 X 10-4 and 9.6 X 10-4. Approximately 70% of the AFB1 bound to DNA was chromatographically identical to 2,3-dihydro-2-(N7-guanyl)-3-hydroxyaflatoxin B1 at the two AFB1 levels used.
AB - Salmonella typhimurium strain TM677 was mutagenized with aflatoxin B1 (AFB1) in liquid suspension culture in the presence of a rat liver postmitochondrial supernatant. Forward mutation to 8-azaguanine resistance was measured in the treated cultures and was found to increase linearly with AFB1 concentration. DNA purified from mutagenized cells was analyzed for AFB1 adduct formation by high-pressure liquid chromatography after adduct liberation. AFB1 exposures at 0.16 and 0.32 μM for 35 min produced 15 and 22 AFB1-DNA adducts per genome, respectively, and induced 8-azaguanine-resistant fractions of 4.9 X 10-4 and 9.6 X 10-4. Approximately 70% of the AFB1 bound to DNA was chromatographically identical to 2,3-dihydro-2-(N7-guanyl)-3-hydroxyaflatoxin B1 at the two AFB1 levels used.
UR - http://www.scopus.com/inward/record.url?scp=0018447818&partnerID=8YFLogxK
U2 - 10.1073/pnas.76.3.1343
DO - 10.1073/pnas.76.3.1343
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AN - SCOPUS:0018447818
SN - 0027-8424
VL - 76
SP - 1343
EP - 1347
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 3
ER -