Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62

Guotai Yu, Oadi Matny, Nicolas Champouret, Burkhard Steuernagel, Matthew J. Moscou, Inmaculada Hernández-Pinzón, Phon Green, Sadiye Hayta, Mark Smedley, Wendy Harwood, Ngonidzashe Kangara, Yajuan Yue, Catherine Gardener, Mark J. Banfield, Pablo D. Olivera, Cole Welchin, Jamie Simmons, Eitan Millet, Anna Minz-Dub, Moshe RonenRaz Avni, Amir Sharon, Mehran Patpour, Annemarie F. Justesen, Murukarthick Jayakodi, Axel Himmelbach, Nils Stein, Shuangye Wu, Jesse Poland, Jennifer Ens, Curtis Pozniak, Miroslava Karafiátová, István Molnár, Jaroslav Doležel, Eric R. Ward, T. Lynne Reuber, Jonathan D.G. Jones, Martin Mascher, Brian J. Steffenson*, Brande B.H. Wulff*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


The wild relatives and progenitors of wheat have been widely used as sources of disease resistance (R) genes. Molecular identification and characterization of these R genes facilitates their manipulation and tracking in breeding programmes. Here, we develop a reference-quality genome assembly of the wild diploid wheat relative Aegilops sharonensis and use positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene Sr62, which has also been transferred to common wheat. This gene encodes a tandem kinase, homologues of which exist across multiple taxa in the plant kingdom. Stable Sr62 transgenic wheat lines show high levels of resistance against diverse isolates of the stem rust pathogen, highlighting the utility of Sr62 for deployment as part of a polygenic stack to maximize the durability of stem rust resistance.

Original languageEnglish
Article number1607
JournalNature Communications
Issue number1
StatePublished - Dec 2022


FundersFunder number
2Blades Foundation
Harold and Adele Lieberman Germplasm Bank
NBI Computing Infrastructure for Science
Gordon and Betty Moore FoundationGBMF4725
University of Minnesota
Biotechnology and Biological Sciences Research CouncilBBS/E/J/000PR9780
Gatsby Charitable Foundation
Human Frontier Science ProgramLT000218/2011-L
Tel Aviv University


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