Adrenalectomy decreases vasoactive intestinal peptide mRNA levels in the rat suprachiasmatic nucleus

I. Gozes*, R. Avidor, E. Giladi, Y. Shani, B. S. McEwen, M. Dussaillant, W. Rostene

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Vasoactive intestinal peptide (VIP) concentrations were shown to be regulated by adrenal steroids. Therefore, we investigated whether adrenal steroids affect VIP mRNA levels, which would suggest an effect on VIP mRNA expression. Adrenalectomy performed on adult male rats resulted in a significant decrease in VIP mRNA in the hypothalamus (from 10.6 ± 0.3 to 3.5 ± 0.2 arbitrary units). In situ hybridization experiments revealed that a major site of VIP mRNA expression in the hypothalamus is the suprachiasmatic nucleus. Indeed, adrenalectomy resulted in an approximate decrease by half in VIP transcripts in this nucleus. However, this decrease was not reversed by replacement treatment with corticosterone or the glucocorticoid agonist, RU28362. Thus, VIP mRNA may be regulated by indirect mechanisms, influenced by the adrenal gland.

Original languageEnglish
Pages (from-to)24-28
Number of pages5
JournalNeuroscience Letters
Volume167
Issue number1-2
DOIs
StatePublished - 14 Feb 1994

Keywords

  • In situ hybridization
  • RNA blot hybridization
  • VIP mRNA

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