ADP/ATP translocator is essential only for anaerobic growth of yeast Saccharomyces cerevisiae

T. Drgoň; L. Šabová; N. Nelson; J. Kolarov

doi:10.1016/0014-5793(91)81059-H

ADP/ATP translocator is essential only for anaerobic growth of yeast Saccharomyces cerevisiae

T. Drgoň
, L. Šabová
, N. Nelson^*
, J. Kolarov

^*Corresponding author for this work

Slovak Academy of Sciences
Hoffmann-La Roche Inc.

Research output: Contribution to journal › Article › peer-review

89 Scopus citations

Abstract

All three genes (AAC1,AAC2 and AAC3) encoding the mitochondrial ADP/ATP translocator, were inactivated in a haploid yeast strain by a gene disruption technique. The triple mutant was still able to grow on fermentable carbon sources but only in the presence of oxygen. Under aerobic conditions neither translocator-protein nor carrier-mediated transport was detected in all mutants in which the AAC2 gene was disrupted. It was further shown that a functional AAC genes product is essential only for anaerobic growth of Saccharomyces cerevisiae but not for growth under derepressed conditions. Under anaerobic conditions a non-detectable amount of AAC3 gene product is sufficient to ensure the cell growth and multiplication.

Original language	English
Pages (from-to)	159-162
Number of pages	4
Journal	FEBS Letters
Volume	289
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(91)81059-H
State	Published - 9 Sep 1991
Externally published	Yes

Keywords

ADP/ATP translocator
Gene disruption
Mitochondrion
Yeast

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10.1016/0014-5793(91)81059-H

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abstract = "All three genes (AAC1,AAC2 and AAC3) encoding the mitochondrial ADP/ATP translocator, were inactivated in a haploid yeast strain by a gene disruption technique. The triple mutant was still able to grow on fermentable carbon sources but only in the presence of oxygen. Under aerobic conditions neither translocator-protein nor carrier-mediated transport was detected in all mutants in which the AAC2 gene was disrupted. It was further shown that a functional AAC genes product is essential only for anaerobic growth of Saccharomyces cerevisiae but not for growth under derepressed conditions. Under anaerobic conditions a non-detectable amount of AAC3 gene product is sufficient to ensure the cell growth and multiplication.",

keywords = "ADP/ATP translocator, Gene disruption, Mitochondrion, Yeast",

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year = "1991",

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doi = "10.1016/0014-5793(91)81059-H",

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T1 - ADP/ATP translocator is essential only for anaerobic growth of yeast Saccharomyces cerevisiae

AU - Drgoň, T.

AU - Šabová, L.

AU - Nelson, N.

AU - Kolarov, J.

PY - 1991/9/9

Y1 - 1991/9/9

N2 - All three genes (AAC1,AAC2 and AAC3) encoding the mitochondrial ADP/ATP translocator, were inactivated in a haploid yeast strain by a gene disruption technique. The triple mutant was still able to grow on fermentable carbon sources but only in the presence of oxygen. Under aerobic conditions neither translocator-protein nor carrier-mediated transport was detected in all mutants in which the AAC2 gene was disrupted. It was further shown that a functional AAC genes product is essential only for anaerobic growth of Saccharomyces cerevisiae but not for growth under derepressed conditions. Under anaerobic conditions a non-detectable amount of AAC3 gene product is sufficient to ensure the cell growth and multiplication.

AB - All three genes (AAC1,AAC2 and AAC3) encoding the mitochondrial ADP/ATP translocator, were inactivated in a haploid yeast strain by a gene disruption technique. The triple mutant was still able to grow on fermentable carbon sources but only in the presence of oxygen. Under aerobic conditions neither translocator-protein nor carrier-mediated transport was detected in all mutants in which the AAC2 gene was disrupted. It was further shown that a functional AAC genes product is essential only for anaerobic growth of Saccharomyces cerevisiae but not for growth under derepressed conditions. Under anaerobic conditions a non-detectable amount of AAC3 gene product is sufficient to ensure the cell growth and multiplication.

KW - ADP/ATP translocator

KW - Gene disruption

KW - Mitochondrion

KW - Yeast

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JO - FEBS Letters

JF - FEBS Letters

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ER -

ADP/ATP translocator is essential only for anaerobic growth of yeast Saccharomyces cerevisiae

Abstract

Keywords

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