ADP/ATP translocator is essential only for anaerobic growth of yeast Saccharomyces cerevisiae

T. Drgoň; L. Šabová; N. Nelson; J. Kolarov

doi:10.1016/0014-5793(91)81059-H

ADP/ATP translocator is essential only for anaerobic growth of yeast Saccharomyces cerevisiae

T. Drgoň, L. Šabová, N. Nelson, J. Kolarov

Research output: Contribution to journal › Article › peer-review

Abstract

All three genes (AAC1,AAC2 and AAC3) encoding the mitochondrial ADP/ATP translocator, were inactivated in a haploid yeast strain by a gene disruption technique. The triple mutant was still able to grow on fermentable carbon sources but only in the presence of oxygen. Under aerobic conditions neither translocator-protein nor carrier-mediated transport was detected in all mutants in which the AAC2 gene was disrupted. It was further shown that a functional AAC genes product is essential only for anaerobic growth of Saccharomyces cerevisiae but not for growth under derepressed conditions. Under anaerobic conditions a non-detectable amount of AAC3 gene product is sufficient to ensure the cell growth and multiplication.

Original language	English
Pages (from-to)	159-162
Number of pages	4
Journal	FEBS Letters
Volume	289
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(91)81059-H
State	Published - 9 Sep 1991
Externally published	Yes

Keywords

ADP/ATP translocator
Gene disruption
Mitochondrion
Yeast

Access to Document

10.1016/0014-5793(91)81059-H

Cite this

@article{6fb22e6b3ed94841b5fd9e34dcba76ef,

title = "ADP/ATP translocator is essential only for anaerobic growth of yeast Saccharomyces cerevisiae",

abstract = "All three genes (AAC1,AAC2 and AAC3) encoding the mitochondrial ADP/ATP translocator, were inactivated in a haploid yeast strain by a gene disruption technique. The triple mutant was still able to grow on fermentable carbon sources but only in the presence of oxygen. Under aerobic conditions neither translocator-protein nor carrier-mediated transport was detected in all mutants in which the AAC2 gene was disrupted. It was further shown that a functional AAC genes product is essential only for anaerobic growth of Saccharomyces cerevisiae but not for growth under derepressed conditions. Under anaerobic conditions a non-detectable amount of AAC3 gene product is sufficient to ensure the cell growth and multiplication.",

keywords = "ADP/ATP translocator, Gene disruption, Mitochondrion, Yeast",

author = "T. Drgo{\v n} and L. {\v S}abov{\'a} and N. Nelson and J. Kolarov",

year = "1991",

month = sep,

day = "9",

doi = "10.1016/0014-5793(91)81059-H",

language = "אנגלית",

volume = "289",

pages = "159--162",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "2",

}

TY - JOUR

T1 - ADP/ATP translocator is essential only for anaerobic growth of yeast Saccharomyces cerevisiae

AU - Drgoň, T.

AU - Šabová, L.

AU - Nelson, N.

AU - Kolarov, J.

PY - 1991/9/9

Y1 - 1991/9/9

N2 - All three genes (AAC1,AAC2 and AAC3) encoding the mitochondrial ADP/ATP translocator, were inactivated in a haploid yeast strain by a gene disruption technique. The triple mutant was still able to grow on fermentable carbon sources but only in the presence of oxygen. Under aerobic conditions neither translocator-protein nor carrier-mediated transport was detected in all mutants in which the AAC2 gene was disrupted. It was further shown that a functional AAC genes product is essential only for anaerobic growth of Saccharomyces cerevisiae but not for growth under derepressed conditions. Under anaerobic conditions a non-detectable amount of AAC3 gene product is sufficient to ensure the cell growth and multiplication.

AB - All three genes (AAC1,AAC2 and AAC3) encoding the mitochondrial ADP/ATP translocator, were inactivated in a haploid yeast strain by a gene disruption technique. The triple mutant was still able to grow on fermentable carbon sources but only in the presence of oxygen. Under aerobic conditions neither translocator-protein nor carrier-mediated transport was detected in all mutants in which the AAC2 gene was disrupted. It was further shown that a functional AAC genes product is essential only for anaerobic growth of Saccharomyces cerevisiae but not for growth under derepressed conditions. Under anaerobic conditions a non-detectable amount of AAC3 gene product is sufficient to ensure the cell growth and multiplication.

KW - ADP/ATP translocator

KW - Gene disruption

KW - Mitochondrion

KW - Yeast

UR - http://www.scopus.com/inward/record.url?scp=0025915949&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(91)81059-H

DO - 10.1016/0014-5793(91)81059-H

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:0025915949

VL - 289

SP - 159

EP - 162

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -

ADP/ATP translocator is essential only for anaerobic growth of yeast Saccharomyces cerevisiae

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this