Abstract
All three genes (AAC1,AAC2 and AAC3) encoding the mitochondrial ADP/ATP translocator, were inactivated in a haploid yeast strain by a gene disruption technique. The triple mutant was still able to grow on fermentable carbon sources but only in the presence of oxygen. Under aerobic conditions neither translocator-protein nor carrier-mediated transport was detected in all mutants in which the AAC2 gene was disrupted. It was further shown that a functional AAC genes product is essential only for anaerobic growth of Saccharomyces cerevisiae but not for growth under derepressed conditions. Under anaerobic conditions a non-detectable amount of AAC3 gene product is sufficient to ensure the cell growth and multiplication.
Original language | English |
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Pages (from-to) | 159-162 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 289 |
Issue number | 2 |
DOIs | |
State | Published - 9 Sep 1991 |
Externally published | Yes |
Keywords
- ADP/ATP translocator
- Gene disruption
- Mitochondrion
- Yeast