Adjustment of protein crystal porosity for biotemplating: Chemical and protein engineering tools

Yariv Wine*, Noa Cohen-Hadar, Shira Lagziel-Simis, Yael Dror, Felix Frolow, Amihay Freeman

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Protein crystals, routinely prepared for the elucidation of protein 3D structures by X-ray crystallography, present an ordered and highly accurate 3D array of protein molecules. Inherent to the 3D arrangement of the protein molecules in the crystal is a complementary 3D array of voids made of interconnected cavities and exhibiting highly ordered porosity. Here we propose and demonstrate feasibility of using chemical and genetic tools to alter protein crystal packing by a series of modifications of targeted sites on protein's surface, enabling the generation of a series of protein crystal biotemplates, all originating from same parent protein.

Original languageEnglish
Title of host publicationPorous Media and Its Applications in Science, Engineering, and Industry - Third International Conference
Pages198-203
Number of pages6
DOIs
StatePublished - 2010
Event3rd International Conference on Porous Media and its Applications in Science, Engineering and Industry - Montecatini, Italy
Duration: 20 Jun 200925 Jun 2009

Publication series

NameAIP Conference Proceedings
Volume1254
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Conference on Porous Media and its Applications in Science, Engineering and Industry
Country/TerritoryItaly
CityMontecatini
Period20/06/0925/06/09

Keywords

  • Biotemplating
  • Chemical modification
  • Crystal packing
  • Porosity
  • Protein crystal
  • Site-directed mutation

Fingerprint

Dive into the research topics of 'Adjustment of protein crystal porosity for biotemplating: Chemical and protein engineering tools'. Together they form a unique fingerprint.

Cite this