Adhesion of candida albicans to epithelial cells - Effect of nikkomycin

Esther Segal; S. Gottlieb; Z. Altboum; Y. Gov; Israela Berdicevsky

doi:10.1111/j.1439-0507.1997.tb00168.x

Adhesion of candida albicans to epithelial cells - Effect of nikkomycin

Esther Segal^*, S. Gottlieb, Z. Altboum, Y. Gov, Israela Berdicevsky

^*Corresponding author for this work

Clinical Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

Abstract

This study investigated the effect of the chitin synthetase inhibitors, the nikkomycins (NZ and NZ+NX), on Candida albicans adhesion to buccal epithelial cells (BECs) in vitro. The effect was expressed in reduced chitin synthetase activity and chitin content of fungal cells. In vitro adhesion assays to BECs of Candida exposed to NZ and NZ + NX revealed reduced adhesion values. Light, scanning and transmission electron microscopy (SEM, TEM) of NZ-treated and untreated microorganisms showed changed fungal morphology and reduced adherence of the treated yeasts. Scanning electron microscopy of NZ-treated C. albicans labelled with gold-conjugated wheatgerm agglutinin (WGA) revealed less labelling than in the untreated organisms. A close contact between the fungus and the epithelial cell at a site with intense (WGA)-gold labelling was noted in TEM experiments. The data point- to the involvement of chitin in the adhesion of C. albicans to epithelial cells.

Original language	English
Pages (from-to)	33-39
Number of pages	7
Journal	Mycoses
Volume	40
Issue number	1-2
DOIs	https://doi.org/10.1111/j.1439-0507.1997.tb00168.x
State	Published - 1997

Keywords

Adhesion
Antifungals
Candida albicans
Nikkomycin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1439-0507.1997.tb00168.x

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title = "Adhesion of candida albicans to epithelial cells - Effect of nikkomycin",

abstract = "This study investigated the effect of the chitin synthetase inhibitors, the nikkomycins (NZ and NZ+NX), on Candida albicans adhesion to buccal epithelial cells (BECs) in vitro. The effect was expressed in reduced chitin synthetase activity and chitin content of fungal cells. In vitro adhesion assays to BECs of Candida exposed to NZ and NZ + NX revealed reduced adhesion values. Light, scanning and transmission electron microscopy (SEM, TEM) of NZ-treated and untreated microorganisms showed changed fungal morphology and reduced adherence of the treated yeasts. Scanning electron microscopy of NZ-treated C. albicans labelled with gold-conjugated wheatgerm agglutinin (WGA) revealed less labelling than in the untreated organisms. A close contact between the fungus and the epithelial cell at a site with intense (WGA)-gold labelling was noted in TEM experiments. The data point- to the involvement of chitin in the adhesion of C. albicans to epithelial cells.",

keywords = "Adhesion, Antifungals, Candida albicans, Nikkomycin",

author = "Esther Segal and S. Gottlieb and Z. Altboum and Y. Gov and Israela Berdicevsky",

year = "1997",

doi = "10.1111/j.1439-0507.1997.tb00168.x",

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AU - Segal, Esther

AU - Gottlieb, S.

AU - Altboum, Z.

AU - Gov, Y.

AU - Berdicevsky, Israela

PY - 1997

Y1 - 1997

N2 - This study investigated the effect of the chitin synthetase inhibitors, the nikkomycins (NZ and NZ+NX), on Candida albicans adhesion to buccal epithelial cells (BECs) in vitro. The effect was expressed in reduced chitin synthetase activity and chitin content of fungal cells. In vitro adhesion assays to BECs of Candida exposed to NZ and NZ + NX revealed reduced adhesion values. Light, scanning and transmission electron microscopy (SEM, TEM) of NZ-treated and untreated microorganisms showed changed fungal morphology and reduced adherence of the treated yeasts. Scanning electron microscopy of NZ-treated C. albicans labelled with gold-conjugated wheatgerm agglutinin (WGA) revealed less labelling than in the untreated organisms. A close contact between the fungus and the epithelial cell at a site with intense (WGA)-gold labelling was noted in TEM experiments. The data point- to the involvement of chitin in the adhesion of C. albicans to epithelial cells.

AB - This study investigated the effect of the chitin synthetase inhibitors, the nikkomycins (NZ and NZ+NX), on Candida albicans adhesion to buccal epithelial cells (BECs) in vitro. The effect was expressed in reduced chitin synthetase activity and chitin content of fungal cells. In vitro adhesion assays to BECs of Candida exposed to NZ and NZ + NX revealed reduced adhesion values. Light, scanning and transmission electron microscopy (SEM, TEM) of NZ-treated and untreated microorganisms showed changed fungal morphology and reduced adherence of the treated yeasts. Scanning electron microscopy of NZ-treated C. albicans labelled with gold-conjugated wheatgerm agglutinin (WGA) revealed less labelling than in the untreated organisms. A close contact between the fungus and the epithelial cell at a site with intense (WGA)-gold labelling was noted in TEM experiments. The data point- to the involvement of chitin in the adhesion of C. albicans to epithelial cells.

KW - Adhesion

KW - Antifungals

KW - Candida albicans

KW - Nikkomycin

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M3 - מאמר

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VL - 40

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JO - Mycoses

JF - Mycoses

SN - 0933-7407

IS - 1-2

ER -

Adhesion of candida albicans to epithelial cells - Effect of nikkomycin

Abstract

Keywords

UN SDGs

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