Adhesion of candida albicans to epithelial cells - Effect of nikkomycin

Esther Segal*, S. Gottlieb, Z. Altboum, Y. Gov, Israela Berdicevsky

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

This study investigated the effect of the chitin synthetase inhibitors, the nikkomycins (NZ and NZ+NX), on Candida albicans adhesion to buccal epithelial cells (BECs) in vitro. The effect was expressed in reduced chitin synthetase activity and chitin content of fungal cells. In vitro adhesion assays to BECs of Candida exposed to NZ and NZ + NX revealed reduced adhesion values. Light, scanning and transmission electron microscopy (SEM, TEM) of NZ-treated and untreated microorganisms showed changed fungal morphology and reduced adherence of the treated yeasts. Scanning electron microscopy of NZ-treated C. albicans labelled with gold-conjugated wheatgerm agglutinin (WGA) revealed less labelling than in the untreated organisms. A close contact between the fungus and the epithelial cell at a site with intense (WGA)-gold labelling was noted in TEM experiments. The data point- to the involvement of chitin in the adhesion of C. albicans to epithelial cells.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalMycoses
Volume40
Issue number1-2
DOIs
StatePublished - 1997

Keywords

  • Adhesion
  • Antifungals
  • Candida albicans
  • Nikkomycin

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