Adhesion and Interaction of Candida albicans with Mammalian Tissues in Vitro and in Vivo

Esther Segal, Hana Sandovsky-Losica

Research output: Contribution to journalArticlepeer-review


This chapter discusses the adhesion and interaction of Candida albicans with mammalian tissues in vitro and in vivo. Adhesion can be visualized in vitro microscopically by mixing exfoliated human or animal epithelial cells, various cell lines, or tissue segments with yeast suspensions. It can also be quantitated by determining the number of adhering yeasts or the number of mammalian cells binding the microorganisms. Adhesion in vitro can also be demonstrated and quantitated by use of radiolabeled yeasts or specific antibodies. This chapter focuses on the most commonly used methods in the studies of in vitro adhesion of Candida to epithelial cells, cell lines, and tissue segments as well as the available in vivo models. Detailed description includes the in vitro methods of adhesion to human buccal and vaginal cells, corneocytes, to HeLa cell lines, and murine gastrointestinal (GI) tissue segments. In vivo interactions include murine models of vaginal infection and GI colonization that eventually lead to systemic disease in naive and compromised animals.

Original languageEnglish
Pages (from-to)439-452
Number of pages14
JournalMethods in Enzymology
Issue numberC
StatePublished - 1995


FundersFunder number
Ontario Ministry of Health
UNDP/World Bank/WHOTDR920223
National Institutes of HealthAI 18841


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