TY - JOUR
T1 - Adhesion and Interaction of Candida albicans with Mammalian Tissues in Vitro and in Vivo
AU - Segal, Esther
AU - Sandovsky-Losica, Hana
N1 - Funding Information:
This work was supported in part by a grant from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR920223) to K. C. Kain and National Institutes of Health Grant AI 18841 to J. I. Ravdin. K. C. Kain is the recipient of a Career Scientist Award from the Ontario Ministry of Health.
PY - 1995
Y1 - 1995
N2 - This chapter discusses the adhesion and interaction of Candida albicans with mammalian tissues in vitro and in vivo. Adhesion can be visualized in vitro microscopically by mixing exfoliated human or animal epithelial cells, various cell lines, or tissue segments with yeast suspensions. It can also be quantitated by determining the number of adhering yeasts or the number of mammalian cells binding the microorganisms. Adhesion in vitro can also be demonstrated and quantitated by use of radiolabeled yeasts or specific antibodies. This chapter focuses on the most commonly used methods in the studies of in vitro adhesion of Candida to epithelial cells, cell lines, and tissue segments as well as the available in vivo models. Detailed description includes the in vitro methods of adhesion to human buccal and vaginal cells, corneocytes, to HeLa cell lines, and murine gastrointestinal (GI) tissue segments. In vivo interactions include murine models of vaginal infection and GI colonization that eventually lead to systemic disease in naive and compromised animals.
AB - This chapter discusses the adhesion and interaction of Candida albicans with mammalian tissues in vitro and in vivo. Adhesion can be visualized in vitro microscopically by mixing exfoliated human or animal epithelial cells, various cell lines, or tissue segments with yeast suspensions. It can also be quantitated by determining the number of adhering yeasts or the number of mammalian cells binding the microorganisms. Adhesion in vitro can also be demonstrated and quantitated by use of radiolabeled yeasts or specific antibodies. This chapter focuses on the most commonly used methods in the studies of in vitro adhesion of Candida to epithelial cells, cell lines, and tissue segments as well as the available in vivo models. Detailed description includes the in vitro methods of adhesion to human buccal and vaginal cells, corneocytes, to HeLa cell lines, and murine gastrointestinal (GI) tissue segments. In vivo interactions include murine models of vaginal infection and GI colonization that eventually lead to systemic disease in naive and compromised animals.
UR - http://www.scopus.com/inward/record.url?scp=0029054091&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(95)53038-X
DO - 10.1016/S0076-6879(95)53038-X
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AN - SCOPUS:0029054091
SN - 0076-6879
VL - 253
SP - 439
EP - 452
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -