TY - JOUR
T1 - Adenovirus E1a interferes with expression of vaccinia viral genes
AU - Strauss, Daphna
AU - Elroy-Stein, Orna
AU - Ehrlich, Rachel
N1 - Funding Information:
This research was supported by the US-Israel Binational Science Fund (BSF), a grant from the Israel Health Ministry and the Israel Association Against Cancer Foundation. The authors are grateful to Dr. Perricaudet (Institut Pasteur) for the Ad12cDNAs, to Dr. A.J. Berk (UCLA) for the v13SAd2E1A and vTFIID, to Dr. A. Lewis (NIH) for the antibodies against Ad12E1A, to Dr. B. Moss (NIAID, NIH) for the fruitful discussions and to Drs. Ken Parker, Carl Hammer, Jorge Ochoa-Carey and John E. Coligan (NIAID, NIH) for the critical reviewing of the manuscript.
PY - 1997/1/15
Y1 - 1997/1/15
N2 - The 12S and 13S cDNAs of the oncogene E1a encoded by the early region of adenovirus 12 (Ad12) were overexpressed using the T7/encephalomyocarditis (EMC)/vaccinia hybrid expression system. The E1a proteins were stable for at least 12 h in monkey epithelial BSC1 cells. The E1a proteins were recognized by a rabbit polyclonal antibody and displayed phosphorylation patterns similar to those displayed by the E1a proteins expressed in Ad12-transformed cells. Expression of E1a proteins by recombinant vaccinia virus led to inhibition of vaccinia viral protein synthesis which was observed as soon as 6 h after infection. This suppression was mediated by both the 12S and the 13S products of Ad12E1a and to a somewhat lesser extent by the 13S product of Ad2E1a. The inhibition of vaccinia virus gene expression resulted in enhanced survival of vaccinia virus-infected cells. These results suggest that the proteins encoded by the E1a sequester a viral or a cellular product(s) that is essential for the expression of vaccinia virus-encoded genes.
AB - The 12S and 13S cDNAs of the oncogene E1a encoded by the early region of adenovirus 12 (Ad12) were overexpressed using the T7/encephalomyocarditis (EMC)/vaccinia hybrid expression system. The E1a proteins were stable for at least 12 h in monkey epithelial BSC1 cells. The E1a proteins were recognized by a rabbit polyclonal antibody and displayed phosphorylation patterns similar to those displayed by the E1a proteins expressed in Ad12-transformed cells. Expression of E1a proteins by recombinant vaccinia virus led to inhibition of vaccinia viral protein synthesis which was observed as soon as 6 h after infection. This suppression was mediated by both the 12S and the 13S products of Ad12E1a and to a somewhat lesser extent by the 13S product of Ad2E1a. The inhibition of vaccinia virus gene expression resulted in enhanced survival of vaccinia virus-infected cells. These results suggest that the proteins encoded by the E1a sequester a viral or a cellular product(s) that is essential for the expression of vaccinia virus-encoded genes.
KW - MHC
KW - Recombinant vaccinia virus
KW - Transcription factor
UR - http://www.scopus.com/inward/record.url?scp=0031030790&partnerID=8YFLogxK
U2 - 10.1016/S0378-1119(96)00614-2
DO - 10.1016/S0378-1119(96)00614-2
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AN - SCOPUS:0031030790
SN - 0378-1119
VL - 184
SP - 279
EP - 284
JO - Gene
JF - Gene
IS - 2
ER -