Adding to the CASeload: unwarranted p53 signaling induced by Cas9

Veronica Rendo, Oana M. Enache, Uri Ben-David

Research output: Contribution to journalComment/debate

Abstract

We investigated the genetic and transcriptional changes associated with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated protein 9 (Cas9) expression in human cancer cell lines. For a subset of cell lines with a wild-type tumor protein TP53 (best known as p53), we detected p53 pathway activation, DNA damage accumulation and emerging p53-inactivating mutations following Cas9 introduction. We discuss the potential implications of our findings in basic and translational research.

Original languageEnglish
Article number1789419
JournalMolecular and Cellular Oncology
DOIs
StatePublished - 2020

Keywords

  • CRISPR
  • Cas9
  • DNA damage response
  • TP53 mutation
  • genome editing
  • p53 pathway

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