ADAMTS-1: A new human ovulatory gene and a cumulus marker for fertilization capacity

Yuval Yung; Ettie Maman; Sarah Konopnicki; Berta Cohen; Masha Brengauz; Irina Lojkin; Mariabeatrice Dal Canto; Rubens Fadini; Jehoshua Dor; Ariel Hourvitz

doi:10.1016/j.mce.2010.07.019

ADAMTS-1: A new human ovulatory gene and a cumulus marker for fertilization capacity

Yuval Yung^*, Ettie Maman, Sarah Konopnicki, Berta Cohen, Masha Brengauz, Irina Lojkin, Mariabeatrice Dal Canto, Rubens Fadini, Jehoshua Dor, Ariel Hourvitz

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

ADAM-metallopeptidase with thrombospondin type 1 motifs-1 (ADAMTS-1) null female mice show impaired follicular development and ovulatory processes. However, ADAMTS-1 expression and function in human normal ovulation and folliculogenesis have not yet been determined. The objective of this study is to study the expression patterns of ADAMTS-1 in human granulosa cells (GCs) obtained from follicles aspirated during in vitro maturation (IVM) and in vitro fertilization (IVF) procedures. We found that ADAMTS-1 expression is a luteinizing hormone/human chorionic gonadotropin (LH/hCG)-induced gene whose expression in the mural GCs directly correlated with antral follicular growth. Interestingly, we were able to show a significant correlation between ADAMTS-1 expression in cumulus cells and the fertilization capacity of the related oocyte. In conclusion, human ADAMTS-1 is an ovulatory gene and its expression is LH/hCG- and follicle-size dependent. The correlation between its expression in cumulus GCs and oocyte fertilization capacity suggests a role for ADAMTS-1 in human cumulus function.

Original language	English
Pages (from-to)	104-108
Number of pages	5
Journal	Molecular and Cellular Endocrinology
Volume	328
Issue number	1-2
DOIs	https://doi.org/10.1016/j.mce.2010.07.019
State	Published - Oct 2010

Keywords

ADAMTS-1
Granulose cells
IVF
IVM
LH/hCG
Ovulation

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title = "ADAMTS-1: A new human ovulatory gene and a cumulus marker for fertilization capacity",

abstract = "ADAM-metallopeptidase with thrombospondin type 1 motifs-1 (ADAMTS-1) null female mice show impaired follicular development and ovulatory processes. However, ADAMTS-1 expression and function in human normal ovulation and folliculogenesis have not yet been determined. The objective of this study is to study the expression patterns of ADAMTS-1 in human granulosa cells (GCs) obtained from follicles aspirated during in vitro maturation (IVM) and in vitro fertilization (IVF) procedures. We found that ADAMTS-1 expression is a luteinizing hormone/human chorionic gonadotropin (LH/hCG)-induced gene whose expression in the mural GCs directly correlated with antral follicular growth. Interestingly, we were able to show a significant correlation between ADAMTS-1 expression in cumulus cells and the fertilization capacity of the related oocyte. In conclusion, human ADAMTS-1 is an ovulatory gene and its expression is LH/hCG- and follicle-size dependent. The correlation between its expression in cumulus GCs and oocyte fertilization capacity suggests a role for ADAMTS-1 in human cumulus function.",

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AU - Dal Canto, Mariabeatrice

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ADAMTS-1: A new human ovulatory gene and a cumulus marker for fertilization capacity

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