TY - JOUR
T1 - Active labour is associated with increased oxidisibility of serum lipids ex vivo
AU - Fainaru, Ofer
AU - Almog, Benny
AU - Pinchuk, Ilya
AU - Kupferminc, Michael J.
AU - Lichtenberg, Dov
AU - Many, Ariel
PY - 2002/8
Y1 - 2002/8
N2 - Objective: As a first step towards evaluating the role of oxidative stress in the process of labour, we tested whether term labour is associated with increased oxidisibility of maternal serum lipids. Design: A controlled prospective study. Setting: Tertiary care centre. Population: Twenty healthy women in active labour and 20 healthy pregnant women not in labour (controls) matched for maternal and gestational age. Methods: Venous blood was drawn from women in both groups. Serum levels of lipid peroxidation products and the kinetics of copper-induced oxidation ex vivo were monitored spectroscopically at 37°C by continuous recording of absorbance at 245 nm. Main outcome measures: Oxidative stress parameters. Results: The initial optical density (OD) at 245 nm, attributed to preformed dienic hydroperoxides and 7-keto-cholesterol (main products of lipid peroxidation), was higher in the labouring group than in the controls (1.30 ± 0.11 vs 1.18 ± 0.09, OD 245 nm, respectively, P < 0.001). The lag phase, reflecting resistance of serum lipids to oxidation, was significantly shorter in the labouring group than in the controls (43.2 ± 1.4 vs 56.2 ± 4.7 min, respectively, P = 0.01). Conclusion: High levels of serum hydroperoxides and decreased resistance of serum lipids to copper-induced peroxidation ex vivo suggest labour to be associated with high oxidative stress. Whether oxidative stress is involved in initiating the labour process or is consequent awaits further studies.
AB - Objective: As a first step towards evaluating the role of oxidative stress in the process of labour, we tested whether term labour is associated with increased oxidisibility of maternal serum lipids. Design: A controlled prospective study. Setting: Tertiary care centre. Population: Twenty healthy women in active labour and 20 healthy pregnant women not in labour (controls) matched for maternal and gestational age. Methods: Venous blood was drawn from women in both groups. Serum levels of lipid peroxidation products and the kinetics of copper-induced oxidation ex vivo were monitored spectroscopically at 37°C by continuous recording of absorbance at 245 nm. Main outcome measures: Oxidative stress parameters. Results: The initial optical density (OD) at 245 nm, attributed to preformed dienic hydroperoxides and 7-keto-cholesterol (main products of lipid peroxidation), was higher in the labouring group than in the controls (1.30 ± 0.11 vs 1.18 ± 0.09, OD 245 nm, respectively, P < 0.001). The lag phase, reflecting resistance of serum lipids to oxidation, was significantly shorter in the labouring group than in the controls (43.2 ± 1.4 vs 56.2 ± 4.7 min, respectively, P = 0.01). Conclusion: High levels of serum hydroperoxides and decreased resistance of serum lipids to copper-induced peroxidation ex vivo suggest labour to be associated with high oxidative stress. Whether oxidative stress is involved in initiating the labour process or is consequent awaits further studies.
UR - http://www.scopus.com/inward/record.url?scp=0242349220&partnerID=8YFLogxK
U2 - 10.1111/j.1471-0528.2002.01494.x
DO - 10.1111/j.1471-0528.2002.01494.x
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AN - SCOPUS:0242349220
SN - 1470-0328
VL - 109
SP - 938
EP - 941
JO - BJOG: An International Journal of Obstetrics and Gynaecology
JF - BJOG: An International Journal of Obstetrics and Gynaecology
IS - 8
ER -