Active and Inactive Factor VII in Dubin‐Johnson Syndrome with Factor‐VII Deficiency, Hereditary Factor‐VII Deficiency and on Coumadin Administration

Summary. Factor VII was purified from a lyophilized powder of PTC complex (Hemoplex, Cutter Laboratories) and an antiserum was prepared in rabbits to the partially‐purified fraction. The antiserum was found to inhibit specifically factor VII of normal plasma and the activity of the purified factor‐VII preparation. Inactive factor‐VII‐like material was detected in the plasma of one out of nine patients with factor‐VII deficiency and the Dubin‐Johnson syndrome (DJS), as well as in the plasma of patients on short‐term Coumadin therapy. No inactive factor VII could be detected in the plasma of patients on prolonged Coumadin therapy or in the plasma of two patients with hereditary factor‐VII deficiency. The methodological problems are discussed.