Activation of Muscarinic Receptors Inhibits Apoptosis in PC12M1 Cells

Liora Lindenboim, Ronit Pinkas‐Kramarski, Mordechai Sokolovsky, Reuven Stein

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract: Previous studies have shown that PC12 cells depend on growth factors for their survival. When deprived of growth factors, the cells undergo a dying process termed “apoptosis” (programed cell death). We show here that muscarinic agonists inhibited the apoptotic death of growth factor‐deprived PC12M1 cells (PC12 cells stably expressing cloned m1 muscarinic acetylcholine receptors). This protective effect of the muscarinic agonists was observed in both proliferating and neuronal PC12M1 cells, was blocked by the muscarinic antagonist atropine, and was not observed in PC12 cells lacking m1 receptors. Muscarinic receptors therefore mediate inhibition of apoptosis in these cells. In addition to its effect on survival, the muscarinic agonist oxotremorine induced inhibition of DNA synthesis as well as growth arrest of exponentially growing PC12M1 cells at the S and G2/M phases of the cell cycle. Muscarinic receptors in these cells may therefore mediate inhibition of cell cycle progression.

Original languageEnglish
Pages (from-to)2491-2499
Number of pages9
JournalJournal of Neurochemistry
Volume64
Issue number6
DOIs
StatePublished - Jun 1995

Keywords

  • Muscarinic receptors
  • PC12 cells
  • Programed cell death

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