Activation of lymphocytes by BAT and anti CTLA-4: Comparison of binding to T and B cells

Annat Raiter, Abraham Novogrodsky, Britta Hardy*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


In this study we compare the binding and immune stimulatory properties of BAT and anti CTLA-4 monoclonal antibodies (mAbs). Both antibodies were previously shown to manifest effective immune responses against tumor cells. We have described that BAT antibody, produced against Daudi, a B lymphoblastoid cell line, binds and activates T cells. In this paper we demonstrate that anti CTLA-4, produced against the T-cell activation determinant CTLA-4, binds also to B lymphoblastoid cell lines like Daudi and Raji. Both antibodies do not bind resting B cells. BAT binds resting T lymphocytes as well as activated T lymphocytes, whereas anti CTLA-4 binds only activated T cells. Competitive binding experiments indicate that the binding sites of BAT and anti CTLA-4 on activated T cells are distinct. We have studied the in vitro stimulatory effect of BAT and anti CTLA-4 on lymphocytes cultured with or without tumor cells. In contrast to BAT that increased the proliferation of lymphocytes that have been cultured with tumor cells, anti CTLA-4 did not synergize with tumor cells to enhance lymphocyte proliferation.

Original languageEnglish
Pages (from-to)247-251
Number of pages5
JournalImmunology Letters
Issue number2
StatePublished - 3 Aug 1999
Externally publishedYes


  • Anti CTLA-4
  • BAT monoclonal antibody
  • Lymphocyte activation


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