Activation of human neutrophil NADPH oxidase and lateral mobility of membrane proteins. A study with crosslinkers

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Abstract

Fluorescence photobleaching recovery was employed to investigate the relationship between the activation of neutrophil NADPH oxidase and lateral mobility of membrane proteins. Treatment of neutrophils with the crosslinking reagent disuccinimidyl suberate (DSS) blocked activation of the respiratory burst without affecting the lateral motion of concanavalin A receptors. Neutrophils treated with DSS after prestimulation with concanavalin A generated superoxide in response to another stimulator, phorbol myristate acetate, in spite of the lateral immobilization of concanavalin A receptors. The apparent lack of correlation between the activation of NADPH oxidase and the lateral motion of membrane proteins is discussed.

Original languageEnglish
Pages (from-to)227-231
Number of pages5
JournalBBA - Molecular Cell Research
Volume805
Issue number2
DOIs
StatePublished - 12 Oct 1984

Keywords

  • (Human neutrophil)
  • Cross-linking
  • Membrane protein mobility
  • NADPH oxidase

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