Activation of exocytosis by the heterotrimeric G protein Gi3

Meir Aridor, Gladys Rajmilevich, Michael A. Beaven, Ronit Sagi-Eisenberg

Research output: Contribution to journalArticlepeer-review

Abstract

Secretagogues of rat peritoneal mast cells, such as mastoparan and compound 48/80, induce mast cell exocytosis by activating directly the guanosine triphosphate-binding proteins that are required for exocytosis. The introduction of a synthetic peptide that corresponds to the carboxyl-terminal end sequence of Gαi3 into the cells specifically blocked this secretion. Similar results were obtained when antibodies to this peptide were introduced. The Gαi3 was located in both the Golgi and the plasma membrane, but only the latter source of Gαi3 appeared to be essential for secretion. These results indicate that Gαi3 functions to control regulated exocytosis in mast cells.

Original languageEnglish
Pages (from-to)1569-1572
Number of pages4
JournalScience
Volume262
Issue number5139
DOIs
StatePublished - 1993
Externally publishedYes

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