TY - JOUR
T1 - Activated T lymphocytes induce degranulation and cytokine production by human mast cells following cell-to-cell contact
AU - Bhattacharyya, Siba P.
AU - Drucker, Ilana
AU - Reshef, Tamar
AU - Kirshenbaum, Arnold S.
AU - Metcalfe, Dean D.
AU - Mekori, Yoseph A.
PY - 1998/3
Y1 - 1998/3
N2 - Activated mast cells reside in close apposition to T cells in some inflammatory processes. In this study, we analyzed whether this close physical proximity affects human mast cell degranulation and cytokine release. Thus HMC-1 human mast cells or primary bone marrow-derived human mast cells were cocultured with activated and with resting T cells. Mast cells cocultured with activated T cells released histamine and β- hexosaminidase and produced tumor necrosis factor α (TNF-α), an effect that peaked at 20 h. Kinetics of histamine release paralleled the formation of heterotypic aggregates. Separation of the two cell populations with a porous membrane prevented mediator release and TNF-α production. Addition of the PI3-kinase inhibitor, wortmannin, inhibited the heterotypic adhesion- associated degranulation but not TNF-α production. These data thus indicate a novel pathway through which human mast cells are activated to both release granule-associated mediators and to produce cytokines in association with heterotypic adhesion to activated human T cells.
AB - Activated mast cells reside in close apposition to T cells in some inflammatory processes. In this study, we analyzed whether this close physical proximity affects human mast cell degranulation and cytokine release. Thus HMC-1 human mast cells or primary bone marrow-derived human mast cells were cocultured with activated and with resting T cells. Mast cells cocultured with activated T cells released histamine and β- hexosaminidase and produced tumor necrosis factor α (TNF-α), an effect that peaked at 20 h. Kinetics of histamine release paralleled the formation of heterotypic aggregates. Separation of the two cell populations with a porous membrane prevented mediator release and TNF-α production. Addition of the PI3-kinase inhibitor, wortmannin, inhibited the heterotypic adhesion- associated degranulation but not TNF-α production. These data thus indicate a novel pathway through which human mast cells are activated to both release granule-associated mediators and to produce cytokines in association with heterotypic adhesion to activated human T cells.
KW - Heterotypic adhesion
KW - Histamine
KW - Tumor necrosis factor α
UR - http://www.scopus.com/inward/record.url?scp=0031908391&partnerID=8YFLogxK
U2 - 10.1002/jlb.63.3.337
DO - 10.1002/jlb.63.3.337
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C2 - 9500521
AN - SCOPUS:0031908391
SN - 0741-5400
VL - 63
SP - 337
EP - 341
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
IS - 3
ER -