Acetylcholinesterase and butyrylcholinesterase genes coamplify in primary ovarian carcinomas

Haim Zakut, Gal Ehrlich, An Ayalon, Catherine A. Prody, Gustavo Malinger, Shlomo Seidman, Dalia Ginzberg, Ralph Kehlenbach, Hermona Soreq

Research output: Contribution to journalArticlepeer-review

Abstract

The genes for acetylcholinesterase (ACHE) and butyrylcholinesterase (CHE) are expressed in multiple tumor tissues, including ovarian carcinomas. Both CHE and ACHE genes coamplify in leukemias. To examine the relationship of gene amplification to the expression of these genes in tumors, ACHE and CHE genes and their expression were studied in primary ovarian carcinomas. DNA blot hybridization demonstrated a significant amplification and mutagenesis of both genes in 6 of 11 malignant tumors studied. This was greater or of the same order of magnitude as the amplification of the oncogenes c-rafi, v-sis, and c-fes in these tumors. No amplification was found in normal ovarian tissues or benign ovarian cysts. Xenopus oocyte microinjections, blot and in situ hybridizations, and immuno- and cytochemical staining revealed translatable CHEmRNA and its active protein product in discrete tumor foci. The frequent coamplification in ovarian carcinomas of ACHE and CHE genes implicates cholinesterases in neoplastic growth and/or proliferation.

Original languageEnglish
Pages (from-to)900-908
Number of pages9
JournalJournal of Clinical Investigation
Volume86
Issue number3
DOIs
StatePublished - Sep 1990

Keywords

  • Cytochemical staining
  • Immunofluorescence
  • In situ hybridization
  • Oncogenes coamplification
  • Xenopus oocyte bioassay

Fingerprint

Dive into the research topics of 'Acetylcholinesterase and butyrylcholinesterase genes coamplify in primary ovarian carcinomas'. Together they form a unique fingerprint.

Cite this