TY - JOUR
T1 - Acetylcholine and phorbol esters inhibit potassium currents evoked by adenosine and cAMP in Xenopus oocytes
AU - Dascal, N.
AU - Lotan, I.
AU - Gillo, B.
AU - Lester, H. A.
AU - Lass, Y.
PY - 1985
Y1 - 1985
N2 - In Xenopus laevis oocytes, adenosine and other purinergic agonists induce a K+-conductance increase that is fully mimicked by intracellular application of cAMP. Acetylcholine suppressed the K+-conductance increase caused by adenosine, by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine, or by intracellular injection of cAMP. This effect of acetylcholine is not mimicked by intracellular injection of Ca2+ or the Ca-mobilizing agent inositol 1,4,5-triphosphate. However, adenosine and cAMP responses are inhibited by 4β-phorbol 12,13-dibutyrate and 4β-phorbol 12-myristate 13-acetate. These results suggest that, in Xenopus oocytes, the muscarinic inhibition of purinergic and cAMP responses is mediated through the activation of the phospholipid-dependent, Ca-activated protein kinase (protein kinase C).
AB - In Xenopus laevis oocytes, adenosine and other purinergic agonists induce a K+-conductance increase that is fully mimicked by intracellular application of cAMP. Acetylcholine suppressed the K+-conductance increase caused by adenosine, by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine, or by intracellular injection of cAMP. This effect of acetylcholine is not mimicked by intracellular injection of Ca2+ or the Ca-mobilizing agent inositol 1,4,5-triphosphate. However, adenosine and cAMP responses are inhibited by 4β-phorbol 12,13-dibutyrate and 4β-phorbol 12-myristate 13-acetate. These results suggest that, in Xenopus oocytes, the muscarinic inhibition of purinergic and cAMP responses is mediated through the activation of the phospholipid-dependent, Ca-activated protein kinase (protein kinase C).
UR - http://www.scopus.com/inward/record.url?scp=0022351393&partnerID=8YFLogxK
U2 - 10.1073/pnas.82.17.6001
DO - 10.1073/pnas.82.17.6001
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AN - SCOPUS:0022351393
SN - 0027-8424
VL - 82
SP - 6001
EP - 6005
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 17
ER -