Acetylcholine- and inositol 1,4,5-trisphosphate-induced calcium mobilization in Xenopus laevis oocytes

Etta Nadler, Boaz Gillo, Yoram Lass, Yoram Oron

Research output: Contribution to journalArticlepeer-review

Abstract

Acetylcholine induces a complex electrical membrane response in Xenopus laevis oocytes. This response is mimicked, and probably mediated by injected inositol 1,4,5-trisphosphate. Oocytes prelabelled with 45Ca released calcium in two phases, the second, slow phase exhibiting first order kinetics of release. Brief exposure of prelabelled oocytes to acetylcholine resulted in a significant increase in the rate of calcium release that returned to control values 2-3 min following the removal of the neurotransmitter. Intracellular injection of inositol 1,4,5-trisphosphate resulted in increased rate of calcium release similar to, but longer than that caused by acetylcholine. Experiments conducted on single oocytes permitted the investigation of the relationship between acetylcholine-induced and inositol 1,4,5-trisphosphate-induced calcium mobilization and the resulting electrical membrane response. Our data reinforce our previous suggestion that inositol 1,4,5-trisphosphate is the intracellular second messenger of the muscarinic membrane electrical response in Xenopus oocytes.

Original languageEnglish
Pages (from-to)208-212
Number of pages5
JournalFEBS Letters
Volume199
Issue number2
DOIs
StatePublished - 21 Apr 1986

Keywords

  • (Xenopus)
  • Ca mobilization
  • Inositol trisphosphate
  • Muscarinic response
  • Oocyte

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