TY - JOUR
T1 - Acetylcholine- and inositol 1,4,5-trisphosphate-induced calcium mobilization in Xenopus laevis oocytes
AU - Nadler, Etta
AU - Gillo, Boaz
AU - Lass, Yoram
AU - Oron, Yoram
N1 - Funding Information:
This work was supportedb y a basic research grant from Tel Aviv University and by grant 84-00322f rom the US-Israel Binational Science Foundation.
PY - 1986/4/21
Y1 - 1986/4/21
N2 - Acetylcholine induces a complex electrical membrane response in Xenopus laevis oocytes. This response is mimicked, and probably mediated by injected inositol 1,4,5-trisphosphate. Oocytes prelabelled with 45Ca released calcium in two phases, the second, slow phase exhibiting first order kinetics of release. Brief exposure of prelabelled oocytes to acetylcholine resulted in a significant increase in the rate of calcium release that returned to control values 2-3 min following the removal of the neurotransmitter. Intracellular injection of inositol 1,4,5-trisphosphate resulted in increased rate of calcium release similar to, but longer than that caused by acetylcholine. Experiments conducted on single oocytes permitted the investigation of the relationship between acetylcholine-induced and inositol 1,4,5-trisphosphate-induced calcium mobilization and the resulting electrical membrane response. Our data reinforce our previous suggestion that inositol 1,4,5-trisphosphate is the intracellular second messenger of the muscarinic membrane electrical response in Xenopus oocytes.
AB - Acetylcholine induces a complex electrical membrane response in Xenopus laevis oocytes. This response is mimicked, and probably mediated by injected inositol 1,4,5-trisphosphate. Oocytes prelabelled with 45Ca released calcium in two phases, the second, slow phase exhibiting first order kinetics of release. Brief exposure of prelabelled oocytes to acetylcholine resulted in a significant increase in the rate of calcium release that returned to control values 2-3 min following the removal of the neurotransmitter. Intracellular injection of inositol 1,4,5-trisphosphate resulted in increased rate of calcium release similar to, but longer than that caused by acetylcholine. Experiments conducted on single oocytes permitted the investigation of the relationship between acetylcholine-induced and inositol 1,4,5-trisphosphate-induced calcium mobilization and the resulting electrical membrane response. Our data reinforce our previous suggestion that inositol 1,4,5-trisphosphate is the intracellular second messenger of the muscarinic membrane electrical response in Xenopus oocytes.
KW - (Xenopus)
KW - Ca mobilization
KW - Inositol trisphosphate
KW - Muscarinic response
KW - Oocyte
UR - http://www.scopus.com/inward/record.url?scp=0022541773&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(86)80481-1
DO - 10.1016/0014-5793(86)80481-1
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AN - SCOPUS:0022541773
SN - 0014-5793
VL - 199
SP - 208
EP - 212
JO - FEBS Letters
JF - FEBS Letters
IS - 2
ER -