Accuracy of in vivo confocal microscopy for diagnosis of basal cell carcinoma: A comparative study between handheld and wide-probe confocal imaging

R. P. Castro, A. Stephens, N. A. Fraga-Braghiroli, M. C. Oliviero, G. G. Rezze, H. Rabinovitz, A. Scope

Research output: Contribution to journalArticlepeer-review

Abstract

Background Reflectance confocal microscopy (RCM) increases specificity of identification of basal cell carcinoma (BCC). A smaller-diameter handheld RCM (HH-RCM) allows better access to limited anatomic locations. Objective To compare accuracy of HH-RCM in identification of BCC to that of traditional wide-probe RCM (TWP-RCM). Methods Patients presenting at least one lesion clinically and dermoscopically suspicious for BCC, were recruited from two dermatology skin cancer clinics. Prior to excision, we attempted to image all lesions with HH-RCM and TWP-RCM using a standardized protocol. RCM images were retrospectively evaluated, jointly by two blinded readers. For purposes of comparative RCM, sensitivity and specificity analysis, we used a threshold of ≥3 RCM criteria to identify BCC, whereby at least one criterion had to be presence of 'dark silhouettes' or 'bright tumor islands'. Results Among 54 lesions imaged with both RCM devices, 45 were biopsy-proven BCCs. Comparison between TWP-RCM vs. HH-RCM was as follows: sensitivity (100% vs. 93%), specificity (78% for both probes), positive predictive value (96% vs. 95%), and negative predictive value (100% vs. 70%) respectively. Notably, both TWP-RCM and HH-RCM demonstrated the presence of 'dark silhouettes' or 'bright tumor islands' in all 45 BCCs. Conclusion Both RCM probes demonstrate high PPV. TWP-RCM shows higher NPV, since its broader field-of-view probably allows more exhaustive search for BCC criteria. The RCM criteria threshold for BCC identification should be further tested.

Original languageEnglish
Pages (from-to)1164-1169
Number of pages6
JournalJournal of the European Academy of Dermatology and Venereology
Volume29
Issue number6
DOIs
StatePublished - 1 Jun 2015

Fingerprint

Dive into the research topics of 'Accuracy of in vivo confocal microscopy for diagnosis of basal cell carcinoma: A comparative study between handheld and wide-probe confocal imaging'. Together they form a unique fingerprint.

Cite this