Accelerated heme synthesis and degradation in transformed fibroblasts

Rivka Mamet, Leonard Leibovici, Yael Teitz, Nili Schoenfeld

Research output: Contribution to journalArticlepeer-review

Abstract

Various parameters of the heme biosynthetic pathway were studied in two cell lines, one nontransformed and the other malignantly transformed ( MLV MS), both replicating at the same rate. Using the above system enabled us to distinguish between phenomena characteristic of the malignant transformation per se and those due to accelerated growth rate. Heme synthesis and degradation as well as the activities of ALAS, ALAD, PBGD, and FC were found to be increased in the transformed cells. However, the concentration of intracellular heme was markedly reduced from 30.4 ± 4.4 pmole/mg protein in nontransformed cells to 10.5 ± 2.6 pmole/mg protein in transformed cells. These observations show that malignant transformation leads to changes in heme metabolism unrelated to growth rate in this cell line.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalBiochemical Medicine and Metabolic Biology
Volume44
Issue number2
DOIs
StatePublished - Oct 1990
Externally publishedYes

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