ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences /631/92/72/1203 /631/92/555 article

Chethan D. Shanthamurthy; Prashant Jain; Sharon Yehuda; João T. Monteiro; Shani Leviatan Ben-Arye; Balamurugan Subramani; Bernd Lepenies; Vered Padler-Karavani; Raghavendra Kikkeri

doi:10.1038/s41598-018-24333-y

ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences /631/92/72/1203 /631/92/555 article

Chethan D. Shanthamurthy, Prashant Jain, Sharon Yehuda, João T. Monteiro, Shani Leviatan Ben-Arye, Balamurugan Subramani, Bernd Lepenies^*, Vered Padler-Karavani, Raghavendra Kikkeri

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses. Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray. The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca²⁺-dependent fashion.

Original language	English
Article number	6603
Journal	Scientific Reports
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-24333-y
State	Published - 1 Dec 2018

Funding

Funders	Funder number
ETN-Immunoshape
European Union H2020 Program	ERC-2016-STG-716220
European Union’s Horizon 2020 research and innovation program
Marie Sklodowska-Curie
Leona M. and Harry B. Helmsley Charitable Trust
National Nanotechnology Initiative
Horizon 2020 Framework Programme	716220, 642870
Department of Science and Technology, Ministry of Science and Technology, India
Department of Science and Technology, Government of West Bengal	SB/S1/C-46/2014
Indian Institute of Science Education and Research Mohali

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Shanthamurthy, C. D., Jain, P., Yehuda, S., Monteiro, J. T., Leviatan Ben-Arye, S., Subramani, B., Lepenies, B., Padler-Karavani, V., & Kikkeri, R. (2018). ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences /631/92/72/1203 /631/92/555 article. Scientific Reports, 8(1), Article 6603. https://doi.org/10.1038/s41598-018-24333-y

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abstract = "Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses. Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray. The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca2+-dependent fashion.",

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Shanthamurthy, CD, Jain, P, Yehuda, S, Monteiro, JT, Leviatan Ben-Arye, S, Subramani, B, Lepenies, B, Padler-Karavani, V & Kikkeri, R 2018, 'ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences /631/92/72/1203 /631/92/555 article', Scientific Reports, vol. 8, no. 1, 6603. https://doi.org/10.1038/s41598-018-24333-y

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AU - Yehuda, Sharon

AU - Monteiro, João T.

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AU - Subramani, Balamurugan

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AU - Kikkeri, Raghavendra

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AB - Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses. Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray. The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca2+-dependent fashion.

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ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences /631/92/72/1203 /631/92/555 article

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